脂联素的球状结构域对3T3-L1脂肪细胞胰岛素抵抗模型AMPK信号通路的影响  

Effect of globular domain of adiponectin on glucose metabolism and its mechanism in 3T3-L1 adipocytes model which have generated IR

作　　者：王彦[1] 陈思思[2] 廉如[1] 高婷婷[1] 陈显久[3] 杨静[1] 

机构地区：[1]山西医科大学第一医院内分泌科,太原030001 [2]临汾市人民医院内分泌科,山西临汾041000 [3]山西医科大学生物化学与分子生物学教研室,太原030001

出　　处：《中国临床药理学杂志》2012年第9期662-664,共3页The Chinese Journal of Clinical Pharmacology

基　　金：山西省科技攻关基金资助项目(20090321104)

摘　　要：目的观察脂联素的球状结构域(gAd)对3T3-L1脂肪细胞胰岛素抵抗(IR)模型AMPK信号通路的影响。方法将3T3-L1前脂肪细胞诱导分化为成熟的脂肪细胞。用软脂酸制备脂肪细胞IR模型,3个浓度的gAd干预已产生IR的3T3-L1脂肪细胞。用葡萄糖氧化酶法,检测培养液中葡萄糖的浓度;用RT-PCR法,检测腺苷酸活化蛋白激酶(AMPK)、脂肪酸分解代谢关键酶肉碱脂酰转移酶I(CPT-I)基因水平的变化;用Western blot法,检测AMPK Thr-172磷酸化水平。结果 gAd可促进3T3-L1脂肪细胞IR模型葡萄糖摄取,可增加3T3-L1脂肪细胞AMPK、CPT-I基因的表达,且与剂量呈正相关(均P=0.000);gAd可增加3T3-L1脂肪细胞AMPK Thr-172磷酸化水平,且与剂量呈正相关(P=0.000)。结论 gAd经AMPK途径,可改善3T3-L1脂肪细胞的IR。Objective To observe the effect of globular domain of adiponectin on glucose metabolism and its mechanism in 3T3-L1 adipocytes model which have generated insuline resistance（IR）.Methods Culturing and differentiating 3T3-L1 adipocyte.IR model of 3T3-L1 adipocytes were prepared with palmitic acid.Intervention the adipocytes which have been generated IR with different concentrations of gAd.The cell culture medium glucose content was detected with the glucose oxidize method.The mRNA expression of AMPK,CPT-I were detected with real-time quantitative PCR method.The phosphorylation of AMPK Thr-172 was detected by Western blot.Results Gad can promote glucose uptake of 3T3-L1 adipocytes model which have generated IR.Different concentrations of gAd gradually increased the mRNA expression levels of AMPK,CPT-I and in a dose-dependent manner.The AMPK Thr-172 phosphorylation levels increased gradually followed by the concentration of gAd（P=0.000）.Conclusion gAd can improve insulin resistance by AMPK signal transduction in 3T3-L1 adipocytes model which have generated IR.

关 键 词：胰岛素抵抗 脂联素球状结构域 3T3-L1脂肪细胞 

分 类 号：R587[医药卫生—内分泌] R966[医药卫生—内科学]