三七总皂苷对糖尿病肾病大鼠的肾脏保护作用及机制  

Experimental study on protective effect and its mechanisms of panax notoginseng saponins in rat with diabetic nephropathy

作　　者：徐刚[1] 刘茂林[1] 傅珍春[1] 范超明[2] 黄妙珍[1] 赖登攀[1] 宁锂[3] 张秋玲[3] 

机构地区：[1]杭州师范大学肾脏病研究所附属医院肾内科,杭州310015 [2]杭州师范大学肾脏病研究所附属医院检验中心,杭州310015 [3]杭州师范大学肾脏病研究所附属医院内分泌科,杭州310015

出　　处：《中国临床药理学杂志》2012年第9期665-667,共3页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省医药卫生科研计划基金资助项目(2003A076);杭州市医药卫生科研计划基金资助项目(2003A009)

摘　　要：目的观察三七总皂苷(PNS)对DN的肾脏保护作用及机制。方法 36只雄性SD大鼠分成正常组、模型组、PNS干预组(均12只)。4,8周末,检测血清和肾皮质的晚期糖基化终产物(AGEs)、超氧化物歧化酶(SOD)、丙二醛(MDA)以及肾皮质VEGF含量。结果 PNS干预组,血清与肾组织的MDA、AGEs和VEGF含量,均明显低于模型组(P<0.01,P<0.05);而SOD显著高于模型组(P<0.05)。结论 PNS可能通过抗氧化、抑制AGEs生成、下调肾组织VEGF表达,来延缓DN大鼠肾脏病变进展。Objective To explore the protective effect of panax notoginseng saponins（PNS） in rat with diabetic nephropathy（DN）.Methods A total of 36 model rats were randomly divided into normal group（n =12）,model group（n = 12） and PNS treatment group（n=12）.The rat DN model was induced by intraperitoneal injection of streptozotocin in the left lower abdomen of the rats.In the experiment the 4th weekend and 8th weekend,serum and renal cortex supperoxide dismutase（SOD） and malondialdehyde（MDA） of the rats in each group were measured by colorimetric assay.Serum and renal tissue advanced glycation end products（AGEs） were determined by spectrum law of fluorescence.The expression of VEGF in renal tissue was observed by the immunohistochemistry.Results In the diabetic animals treated with PNS,AGEs and MDA in serum and renal tissue and VEGF of renal tissue were lower than those in diabetic animals（P〈0.01 or P〈0.05）,and the SOD in serum and renal tissue were increased obviously（P〈0.05）.Conclusion PNS can slow down renal disease progression in DN rats through anti-oxidant,inhibiting overproduction of AGEs and downregulating the expression of VEGF in renal tissue.

关 键 词：糖尿病肾病 氧化应激 血管内皮细胞生长因子 三七总皂苷 

分 类 号：R285.5[医药卫生—中药学] R977.9[医药卫生—中医学]