脂多糖对正常人皮肤成纤维细胞胶原合成的影响  

Influence of lipopolysaccharide on the collagen synthesis of normal human skin fibroblasts

作　　者：纪少军[1] 万立[1] 李凤玉[1] 贾国洪[1] 闫永宏[1] 石军[1] 

机构地区：[1]解放军第二五一医院急诊科,河北张家口075000

出　　处：《东南大学学报（医学版）》2012年第5期613-615,共3页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(30371467)

摘　　要：目的:研究脂多糖(lipopolysaccharide,LPS)对正常人皮肤成纤维细胞胶原合成的影响,以阐明LPS在皮肤创伤愈合中的可能作用。方法:体外培养正常人皮肤成纤维细胞,采用3H-脯氨酸掺入法观察不同浓度(0、0.005、0.01、0.05、0.1、0.5和1.0μg.ml-1)的LPS对成纤维细胞胶原合成的影响。结果:LPS刺激浓度在0.005μg.ml-1时,皮肤成纤维细胞胶原蛋白合成增加;随着刺激浓度增加,刺激作用增强;但当LPS浓度为0.5μg.ml-1时,促胶原合成作用开始降低;当浓度达到1.0μg.ml-1时则呈现抑制效应。结论:在一定浓度范围内LPS促进成纤维细胞胶原合成,表明LPS在皮肤创伤愈合中可能具有重要作用。Objective： To investigate the influence of lipopolysaccharide（LPS） on the collagen synthesis of normal human skin fibroblasts and its relation with wound healing.Methods： Normal skin fibroblasts were exposed to LPS for a certain period of time.Then the collagen synthesis of fibroblasts in culture medium was measured with the method of pepsin digestion after incorporation of 3H-proline into stable,single-layered,confluent fibroblasts.Results： Collagen synthesis of normal skin fibroblasts increased when challenged with LPS at the concentration of 0.005 μg·ml-1,and the increase showed a concentration dependent manner.However,when the concentration of LPS was 0.5 μg·ml-1,the influence of LPS on the collagen synthesis of normal skin fibroblasts began to decrease;when the concentration of LPS reached 1.0 μg·ml-1,collagen synthesis of fibroblasts were inhibited.Conclusion： LPS could promote collagen synthesis of normal human skin fibroblasts within a certain extent,suggesting that LPS might play an important role in wound healing.

关 键 词：脂多糖 胶原 成纤维细胞 创面愈合 

分 类 号：Q516[生物学—生物化学] R335[医药卫生—人体生理学]