黄芪和三七主要有效成分配伍对氧化损伤所致的PC12细胞凋亡及其活性氧、线粒体膜电位的影响  

Effects of the combination of active component extracts from Astragalus membranaceus and Panax notoginseng on apoptosis, reactive oxygen species and mitochondrial membrane potential of PC12 cells with oxidative injury

作　　者：黄小平[1] 刘晓丹[2] 邓常清[2] 

机构地区：[1]湖南中医药大学医学院,湖南长沙410208 [2]湖南中医药大学中西医结合学院,湖南长沙410208

出　　处：《中西医结合学报》2012年第10期1127-1134,共8页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.81102557);教育部2010年度高等学校博士学科点专项科研基金资助项目(No.20104323110001);湖南省高校创新平台开放基金资助项目(No.11K050);湖南省教育厅一般项目(No.11C0963);湖南省高校科技创新团队支持计划

摘　　要：目的:探讨黄芪主要有效成分黄芪甲苷和三七主要有效成分人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1配伍抗氯化钴(cobalt chloride,CoCl2)诱导的PC12细胞氧化损伤的作用及其机制。方法:应用CoCl2诱导经神经生长因子转分化的PC12细胞氧化损伤模型,经不同药物处理后,采用Hocchst33258荧光染色检测细胞凋亡,罗丹明123荧光染色检测细胞线粒体膜电位(mitochondrial membrane potential,MMP),2′,7′-二氯荧光黄双乙酸盐荧光染色检测细胞内活性氧(reactive oxygen species,ROS)水平。结果:CoCl2能引起转分化后的PC12细胞凋亡,同时PC12细胞MMP水平显著降低,ROS生成显著增加。黄芪甲苷、人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1均能不同程度地抑制CoCl2诱导的PC12细胞凋亡,减少损伤后ROS的生成和MMP的下降,4个有效成分配伍的效应强于各有效成分单用。结论:黄芪和三七的主要有效成分能抑制氧化损伤后的PC12细胞凋亡,有效成分配伍后作用加强,其机制可能与抑制细胞活性氧的生成,提高线粒体膜电位有关。To explore the effects and mechanisms of combining astragaloside Ⅳ（the effective component of Astragalus membranaceus） with notoginsenoside R1, ginsenoside Rb1 and ginsenoside Rg1 （the effective components of Panax notoginseng） against oxidative injury in PC12 cells induced by cobalt chloride （CoCl2）. METHODS： CoCl2 was used to stimulate PC12 cells to induce injury after transdifferentiation with nerve growth factor. Then the PC12 cells were divided into 10 groups and cultured with corresponding drugs. After culture, apoptotic cells were tested by using Hocchst 33258 fluorescent staining, the level of mitochondrial membrane potential （MMP） was analyzed by rhodamine 123 fluorescent staining and the content of reactive oxygen species （ROS） in PC12 cell was measured by dichlorofluorescin diacetate fluorescent staining. RESULTS： CoCl2 induced apoptosis along with the obvious decrease of MMP as well as overproduction of ROS in PC12 cells. Astragaloside Ⅳ, ginsenosides Rgl, ginsenosides Rbl and notoginsenoside R1 had inhibition effects in different degree on PC12 cell apoptosis induced by CoCl2, reduced the overproduction of ROS and the decrease of MMP. The effects of the combination were better than those of active component alone. CONCLUSION： Active components extracted from Astragalus and Panax notoginseng can inhibit PC12 cell apoptosis induced by oxidative injury, furthermore, the effects were enhanced by combination of these components, which may be associated with jointly antagonizing the generation of ROS and raising MMP.

关 键 词：植物提取物 中药配伍 细胞凋亡 活性氧 线粒体膜电位 PC12细胞 

分 类 号：R285[医药卫生—中药学]