子宫内膜异位症患者外周血CD4^+CD25^+Foxp3^+调节性T细胞的变化  

The changes of CD4^+CD25^+Foxp3^+ regulatory T cells in the peripheral blood of patients with endometriosis

作　　者：翁翠萍[1] 郑元秀[1] 谢中华[1] 

机构地区：[1]浙江省杭州市余杭区第二人民医院,杭州311121

出　　处：《现代免疫学》2012年第5期421-424,共4页Current Immunology

摘　　要：观察子宫内膜异位症患者外周血单个核细胞CD4^+CD25^+Foxp3^+调节性T细胞的数量变化,初步探讨其意义。采用流式细胞术检测20例健康对照者(对照组)及46例子宫内膜异位症患者(疾病组临床r-AFS分期:Ⅰ～Ⅱ期26例,Ⅲ～Ⅳ期20例)外周血单个核细胞(PBMC)中CD4^+CD25^+Foxp3^+调节性T细胞数目,并计算CD4^+CD25^+Foxp3^+调节性T细胞占CD4^+T淋巴细胞的百分率;分析不同分期子宫内膜异位症患者外周血CD4^+CD25^+Foxp3^+调节性T细胞的变化。结果显示,与健康对照组相比,疾病组PBMC中CD4^+CD25^+Foxp3^+Treg占CD4^+T淋巴细胞的百分率及CD4^+CD25^+Foxp3^+Treg绝对数均明显升高(P<0.01,P<0.05);疾病组中,Ⅲ～Ⅳ期的PBMC中CD4^+CD25^+Foxp3^+Treg占CD4^+T淋巴细胞的百分比及CD4^+CD25^+Foxp3^+Treg绝对值较Ⅰ～Ⅱ期均明显升高(P<0.01,P<0.05)。提示子宫内膜异位症患者外周血CD4^+CD25^+Foxp3^+调节性T细胞数目和比例增多,可能存在自身免疫调节功能的紊乱,且与病程发展紧密相关。To investigate the changes of CD4＋ CD25＋ Foxp3＋ regulatory T cell in peripheral blood mononuclear cells（PBMC） of endometriosis patients and to study their significance. The CD4＋ CD25＋ Foxp3＋ regulatory T cells of peripheral blood mononu clear cell in 46 patients with endometriosis（r-AFS stage： Ⅰ～Ⅱ stage 26, Ⅲ～Ⅳstage 20） and 20 normal healthies were detec ted by flow cytometry. The differences were statistically analyzed. Compared with healthy controls the proportion and number of CD4＋ CD25＋ Foxp3＋ regulatory T cells in the PBMC of the endometriosis patients were increased significantly（P〈0.01, P〈0.05 respectively）, and these phenomena were more significant in the Ⅲ～ IV stages than in the Ⅰ～Ⅱ stages（P〈0.01, P〈 0.05 respectively）. Higher proportion and absolute number of CD4＋ CD25＋ Foxp3＋ regulatory T cell are observed in the PBMC of endometriosis patients and are related to the staging of the disease, suggesting the immune function of endometriosis patients may be compromised.

关 键 词：子宫内膜异位症 调节性T细胞 流式细胞术 

分 类 号：R711.71[医药卫生—妇产科学]