徐长卿对三硝基苯磺酸诱导的大鼠结肠炎的作用  

Radix cynanchi paniculati ameliorates 2,4,6-trinitrobenzenesulfonic acid-induced colitis in rats

作　　者：贺海辉 沈洪 朱宣宣 顾培青 刘亚军 朱磊 郑凯 

机构地区：[1]南京中医药大学附属医院消化科 国家中医(脾胃病)临床研究基地,江苏省南京市210029 [2]南京中医药大学附属医院药理实验室,江苏省南京市210029

出　　处：《世界华人消化杂志》2012年第24期2237-2242,共6页World Chinese Journal of Digestology

基　　金："十一五"国家科技支撑计划基金资助项目;No.2006BAI04A14;国家自然科学基金资助项目;No.81072778~~

摘　　要：目的:探讨徐长卿对2,4,6-三硝基苯磺酸(trinitrobenzenesulfonic acid,TNBS)诱导的大鼠结肠炎的作用.方法:将40只♂SD大鼠随机分为4组:正常组、模型组、徐长卿组和巴柳氮组.除正常组外,其余3组大鼠均以TNBS灌肠造模.灌肠24h后,徐长卿组开始每天给予徐长卿4g/kg;巴柳氮组给予巴柳氮钠1g/kg灌胃治疗10d.每天观察大鼠一般情况,给药结束后,观察大鼠结肠大体损伤及病理,酵素免疫分析法(enzyme-linked immunosorbant assay,ELISA)检测肠组织肿瘤坏死因子(tumor necrosis factor,TNF)-α、白介素(interleukin,IL)-1β及IL-10水平.结果:两治疗组体质量较模型组增加,但差异无统计学意义;两治疗组疾病活动指数(disease activity index,DAI)评分较模型组明显下降(0.70±1.06,0.67±0.71vs2.38±1.51,P<0.05).徐长卿组、巴柳氮组结肠大体损伤及病理评分较模型组显著下降(1.05±0.83,1.06±0.85vs2.94±0.94;1.65±1.67,2.00±1.80vs6.00±1.67,均P<0.01).徐长卿组较模型组TNF-α、IL-1β水平明显降低(P<0.01),IL-10水平无统计学差异.巴柳氮组较模型组TNF-α、IL-1β、IL-10水平均明显降低(P<0.01).结论:徐长卿能有效改善TNBS诱导的大鼠结肠炎,其机制可能与调节细胞因子水平有关.AIM： To investigate the effects of radix cynanchi paniculati in the treatment of 2,4,6-trinitroben- zenesulfonic acid （TNBS）-induced colitis in rats.METHODS： Forty male Sprague-Dawley （SD）rats were randomly divided into four groups： control group, model group, radix cynanchi paniculati group, and balsalazide group. Experi- mental colitis was induced in rats by intragastric administration of TNBS. Rats in the radix cy- nanchi paniculati and balsalazide groups were treated by gavage with radix cynanchi paniculati （4 g/kg） or balsalazide （1 g/kg） for ten consecu- tive days following TNBS administration. After treatment, general status, macroscopic lesions and histological injuries were observed, and serum levels of cytokines, including interleukin （IL）-1β, tumor necrosis factor （TNF）-α and IL-10 were determined by enzyme-linked immunosor- bant assay （ELISA）. RESULTS： Body weight was improved in rats of the two treatment groups, but there was no statistical difference in body weight between the treatment groups and model group. Disease activity index （DAI） was significantly lower in the two treatment groups than in the model group （0.70 ± 1.06, 0.67 ± 0.71 vs 2.38 ± 1.51, both P 〈 0.05）. Both radix cynanchi paniculati and balsalazide could improve colonic macroscopic morphology and pathohistology compared to the model group （1.05 ± 0.83, 1.06 ± 0.85 vs 2.94 ± 0.94; 1.65 ± 1.67, 2.00 ± 1.80 vs 6.00 ± 1.67, all P 〈 0.01）. Serum levels of TNF-α and IL-1β were sig- nificantly lower in the radix cynanchi paniculati group than in the model group （both P 〈 0.01）, but there was no statistical difference in IL-10 level between the two groups. Serum levels of TNF-α, IL-1β and IL-10 were significantly lower in the balsalazide group than in the model group （all P 〈 0.01）. CONCLUSION： Radix cynanchi paniculati could effectively ameliorate TNBS-induced colitis in rats possibly via mechanisms associated with altering the levels of cytokines.

关 键 词：徐长卿 2 4 6-三硝基苯磺酸 结肠炎 细胞因子 

分 类 号：R285.5[医药卫生—中药学]