当归水醇提取物对哮喘患者血CD4^+CD25^+T调节细胞IFN-γ表达的影响  

The influence of angelica sinensis on IFN-γ in the CD4^+CD25^+T regulatory cells isolated from asthma patients

作　　者：罗江秀 钟超 

机构地区：[1]深圳市光明新区人民医院药剂科,广东深圳518106

出　　处：《中国现代医生》2012年第27期45-47,共3页China Modern Doctor

摘　　要：目的研究当归水醇提取物对哮喘患者血CD4+CD25+T调节细胞IFN-γ表达的影响。方法分离50例哮喘患者的外周静脉血CD4+CD25+T调节细胞,采用Western blot研究不同浓度(3.13 ng/mL,6.25 ng/mL,12.5 ng/mL,25 ng/mL)的当归水醇提取物对CD4+CD25+T细胞IFN-γ表达情况以及25 ng/mL的当归水醇提取物处理CD4+CD25+T细胞不同时间(24 h、36 h、48 h)后IFN-γ表达情况。结果随着当归水醇提取物浓度的增加,CD4+CD25+T调节细胞IFN-γ表达增加。随着当归水醇提取物处理的时间延长,CD4+CD25+T调节细胞IFN-γ表达增加。结论当归水醇提取物浓度对哮喘患者IFN-γ表达呈现明显的时间-浓度依赖关系,提示当归水醇提取物可能通过影响IFN-γ表达来影响哮喘的发生。Objective To identify the influence of angelica sinensis on INF-γ in the CD4＋D25＋ T regulatory cells isolated from asthma patients. Methods Different concentration of angelica sinensis （3.13 ng/mL, 6.25 ng/mL, 12.5 ng/mL, 25 ng/mL） effected on asthma patients＇ CD4＋D25＋ T regulatory ceils and different times of angelica sinensis （24 h, 36 h, 48 h） effected on asthma patients＇ CD4＋CD25＋ T regulatory cells, and used Western blot to detect the expression of IFN-γ in CD4＋ CD25＋T regulatory ceils. Results The extracts from angelica sinensis could effect on human CD4＋CD25＋ T regulatory cells and the expression of IFN-γ was increased. Conclusion The extracts from angelica sinensis can infect the expression of IFN-γ in human CD4＋CD25＋ T regulatory cells.

关 键 词：当归水醇提取物 IFN-Γ CD4+CD25+T调节细胞 哮喘 

分 类 号：R285.6[医药卫生—中药学]