Cramp基因敲除对小鼠骨髓造血干细胞的影响  

Influence of Cramp Knockout on the Function of Hematopoietic Stem Cell

作　　者：石桂英[1] 白琳[1] 鞠振宇[1] 

机构地区：[1]中国医学科学院北京协和医学院实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021

出　　处：《中国比较医学杂志》2012年第9期13-15,20,共4页Chinese Journal of Comparative Medicine

基　　金：中央级公益性科研院所基本科研业务费(DWS201010)

摘　　要：目的研究Cramp基因敲除在衰老过程中对小鼠造血干细胞的作用。方法应用流式细胞仪分析3月龄及12月龄Cramp基因敲除小鼠及同窝野生型小鼠的骨髓造血干细胞的比例及不同发育阶段B淋巴细胞的比例。结果与野生型小鼠相比,12月龄Cramp基因敲除小鼠的骨髓长期造血干细胞增多,多潜能造血祖细胞减少;前体B淋巴细胞和未成熟B淋巴细胞减少,成熟B淋巴细胞增多。结论在衰老过程中,Cramp基因敲除对骨髓造血干细胞及B淋巴细胞发育有重要影响。Objective To study the influence of Cramp gene knockout on the function of hematopoietic stem cell in aging. Methods Cells from the bone marrow of different age Cramp knockout and wild type mice were analyzed by flow cytometry. Results Compared with 12 months old wild type mice, percentage of long term hematopoietic stem cell decreased, multipotent hematopoietic progenitor increased; percentages of pre B and immature B ceils decreased, and mature B cells increased in Cramp knockout mice. Conclusion Cramp gene knockout plays an important role in hematopoietic stem cell and the development of B lymphocytes in aging.

关 键 词：Cramp基因敲除小鼠 流式细胞术 骨髓造血干细胞 B淋巴细胞 

分 类 号：R332[医药卫生—人体生理学]