盐敏感性高血压大鼠eNOS、iNOS表达与心肌细胞凋亡关系分析  

Studies on the Expression of eNOS,iNOS and Heart Apoptosis in Salt-Sensitive Hypertensive Rat

作　　者：殷丽天[1] 黄幸 李莉[1] 张娟[1] 王文娟[1] 杨建一[1] 

机构地区：[1]山西医科大学基础医学院,太原030001 [2]江西省分宜人民医院,新余336600

出　　处：《中国比较医学杂志》2012年第9期41-45,I0003,共6页Chinese Journal of Comparative Medicine

基　　金：山西省自然科学基金项目(200811076-1)

摘　　要：目的探讨盐敏感性高血压形成和心肌细胞损害产生的机制。方法以辣椒辣素损伤Wistar大鼠感觉神经,饲喂高盐饲料,建立盐敏感性高血压大鼠模型。苏木素—伊红染色观察大鼠组织病理学改变;分光光度法检测心肌组织iNOS活性和NO含量;免疫组织化学方法检测心肌eNOS、iNOS蛋白表达;RT-PCR检测心肌eNOS、iNOS mRNA的表达。单细胞凝胶电泳检测心肌细胞凋亡。结果实验结束时各组比较体重无显著性差异(P>0.05)。在第2、3、4周时,辣椒辣素高盐组鼠尾收缩压与对照组相比差异显著(P<0.05)。辣椒辣素高盐组心肌细胞排列紊乱、细胞间隙明显增大,细胞核排列不整齐;心肌iNOS、NO水平升高(P<0.05);eNOS蛋白表达减少(P<0.05)与eNOS mRNA表达减少(P<0.01);iNOS蛋白表达和iNOSmRNA表达显著增高(P<0.01);凋亡细胞数升高(P<0.05)。结论 eNOS mRNA和蛋白的低表达与感觉神经损伤性盐敏感性高血压大鼠形成相关。iNOS mRNA和蛋白的高表达及iNOS活性升高使心肌组织局部产生大量NO。NO可能使得感觉神经损伤性盐敏感性高血压大鼠心肌细胞凋亡增加,从而加重心肌的损伤。Objective To propose the formation of salt-sensitive hypertension and heart cell damage. Methods Wistar rats, capsaicin was injected and high salt diet was feeded, the model of salt-sensitive hypertension was established. the change of heart histopathology were observed, spectrophotometry to detect heart iNOS activity and NO content; immunohistochemistry was performed to detect the expression of eNOS and iNOS protein in heart, the expression of eNOS and iNOS mRNA in heart was determined by RT-PCR, the level of apoptosis was assessed by single cell gel electrophoresis. Results The rats body weight was not significant difference among groups at the end of the experiment （P 〉 0.05）. The capsaicin-high salt diet group systolic blood pressure was significantly higher than the other three groups （P 〈0.05） in 2, 3, 4 weeks. Capsaicin-high salt diet group cardiac muscle cell hypertrophy, muscle fiber disarrangement, nuclei arranged in irregular. The activity of iNOS and level of NO in capsaicin-high salt diet group significantly were higherwhen compared with control group, eNOS protein and eNOS mRNA significantly decreased compared with control group （P 〈 0. 01 ） iNOS protein and iNOS mRNA of capsaicin-high salt diet group were significantly increased compared with control group （P 〈 0.01 ） in heart. The number of apoptotic cells of capsaiein-high salt diet group significantly increased compared with control group （P 〈 0. 05）. Conelttsions The decreased express of eNOS mRNA and protein was related with the forming of salt-sensitive hypertension. The increased express of iNOS mRNA and protein could produce a large number of NO in heart. NO may make the cell apoptosis increasing and the damage of heart in salt-sensitive hypertensive rats.

关 键 词：盐敏感性高血压 辣椒辣素 ENOS INOS 细胞凋亡 

分 类 号：R332[医药卫生—人体生理学]