宫内生长受限对胎猪肝脏microRNA表达谱的影响  

Intrauterine Growth Restriction Alters the Hepatic MicroRNA Expression in Fetal Pigs

作　　者：刘闯[1] 曹中明[2] 林刚[1] 朱玉华[1] 王军军[1] 

机构地区：[1]中国农业大学动物科技学院,动物营养学国家重点实验室,北京100193 [2]四川省达县畜牧食品局,四川达县635006

出　　处：《中国畜牧杂志》2012年第19期42-46,共5页Chinese Journal of Animal Science

基　　金：国家自然科学基金(u0731001);教育部新世纪优秀人才培养资助计划(NCET-08-0530)

摘　　要：本试验旨在分析宫内生长受限(IUGR)和正常胎猪肝脏microRNA(miRNA)表达的差异,以揭示IUGR对胎猪肝脏miRNA表达谱的影响。试验选用6头达兰(Dalland)母猪,在孕110 d左右致死,剖腹并取出整个子宫。在每窝中选取1头IUGR胎猪和1头正常胎猪,分别取出肝脏备用。利用双荧光标记miRNA芯片技术对IUGR和正常胎猪肝脏进行分析。结果表明:IUGR对胎猪肝脏miRNA的表达产生显著影响,共有41种miRNA存在表达差异,其中27种在IUGR胎猪肝脏中表达上调,14种表达下调。其所导致的胎猪葡萄糖吸收代谢紊乱、脂肪代谢紊乱、蛋白质合成受阻;肝脏解毒能力下降以及肝脏细胞增殖抑制,可能是造成IUGR仔猪出生后营养利用率低、患病率和死亡率高以及生长发育不良的重要原因。The present experiment was conducted to reveal the affect of IUGR on hepatic microRNA（miRNA） expression in 110-days-pregnant-fetal pig by using miRNA microarray analysis,in order to advance our knowledge about metabolic programming of intrauterine growth restriction（IUGR） piglet.In this study,six primiparous Dalland sows fed corn and soybean meal-based diet were used.At 110 days of gestation,sows were killed and the whole uterus was re moved.For each sow,one IUGR fetus（with a body weight that is two standard deviations less than the average litter body weight） and one normal-weight fetus were obtained and the left lobe of liver was rapidly sampled and stored at 80℃.Two color miRNA array technology was used to detect the differentially expressed miRNA in the liver between I UGR and normal fetal pig.Our results indicated that,IUGR has significant affect on hepatic miRNA expression in fe tal pig.There are totally 41 differentially expressed miRNA（27 up regulated and 14 down regulated）.These miRNAs ’ targeted genes are involved in the absorption and metabolism of nutrient,hepatic detoxification,hepatic cell growth and proliferation.The disturbed effects of the absorption and metabolism of glucose,the metabolism of lipid,protein synthesis;the decreased ability of hepatic detoxification;and the inhibition of hepatocyte’s proliferation may be an im portant reason responsible for postnatal inefficient nutrient utilization,higher morbidity and mortality,as well as retard ed growth in IUGR piglet.

关 键 词：IUGR 肝脏 MIRNA 胎猪 

分 类 号：S828.5[农业科学—畜牧学]