丹参对肝星状细胞间质胶原酶及I型胶原基因表达的调节  

Regulation of salvia miltiorrhiza on gene expression of interstitial collagenase and type I collagen in HSC-T6-cell

作　　者：王爱民[1] 阴赪宏[1] 朱志坚[1] 马雪梅[1] 付晓霞[1] 王宝恩[1] 

机构地区：[1]解放军252医院消化科,河北保定071000

出　　处：《中华实验外科杂志》2012年第10期1938-1939,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察丹参对HSC-T6细胞间质胶原酶及I型胶原基因表达的影响。方法以不同浓度的丹参（0．8、0．4g／L）作用于培养的肝星状细胞株HSC-T6细胞48h 收集细胞，提取总RNA；用逆转录-聚合酶链反应（RT—PCR）方法测定间质胶原酶（MMP13）及I型胶原的基因表达水平。结果MMP13基因表达水平在丹参0．8、0．4g／L两组分别为0．25±0．05、0．27±0．06，而空白对照组为0．26±0．04。I型胶原基因表达水平在丹参0．8、0．4g／L两组分别为0．76±0．10、1．55±0．23，而空白对照组为3．12±0．46。结论丹参可明显抑制HSC-T6细胞I型胶原的基因表达，且与剂量有关；但对间质胶原酶的基因表达无明显影响。Objective To investigate the effects of salvia miltiorrhiza on gene expression of interstitial collagenase and type I collagen in HSC-T6 cell. Methods Hepatic stellate cell （ HSC-T6 ） was cultured in medium containing different concentration of salvia mihiorrhiza （0. 8, 0. 4 g/L） and cells were collected after＂ incubation for 48 hours. Total RNA of HSC was isolated and gene expression levels of interstitial collagenase （MMP13） and type I c？llagen were measured by reverse transcription polymerase chain reaction. Results MMP13 gene expression level of different groups of salvia mihiorrhiza （0. 8, 0. 4 g/L） were 0. 25 ＋ 0. 05, 0. 27 ± 0. 06 respectively and that of control group was 0. 26 ± 0. 04. Type I collagen gene expression level of different groups of salvia mihiorrhiza （0. 8, 0. 4 g/L） were 0. 76 ＋0. 10, 1.55± 0.23 respectively and that of control group was 3.12 ±0.46. Conclusion Salvia miltiorrhiza may significantly inhibit type I collagen gene expression of HSC-T6 and the effects were correlate with the dosage. Sal- via mihiorrhiza has no effect on MMP13 gene expression.

关 键 词：丹参 HSC-T6细胞 间质胶原酶 I型胶原 基因表达 

分 类 号：R285[医药卫生—中药学]