瘦素对大鼠肝脏载脂蛋白M、载脂蛋白AI、载脂蛋白B和低密度脂蛋白受体表达的影响  

Effects of leptin on the expressions of apolipoprotein M, apolipoprotein AI, lipoprotein B and low density fipoprotein receptor on rat liver

作　　者：狄冬梅[1] 张晓膺[1] 罗光华[2] 郑璐[1] 蒋波[1] Peter Nilsson-Ehle 徐宁[3] 

机构地区：[1]苏州大学附属第三医院心胸外科,常州213003 [2]苏州大学附属第三医院综合实验室,常州213003 [3]瑞典隆德大学医院实验医学研究所临床化学系

出　　处：《中华实验外科杂志》2012年第10期1957-1959,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30972955、81071414）

摘　　要：目的观察瘦素对正常SD大鼠肝脏载脂蛋白M（ApoM）、载脂蛋白AI（ApoAI）、载脂蛋白B（ApoB）和低密度脂蛋白受体（LDL—R）表达的影响。方法通过给健康SD大鼠尾静脉置管，微量注射泵持续泵入瘦素的方法建立高瘦素模型，对照组泵人生理盐水。6h后检测大鼠肝脏ApoM表达的变化，以及血清ApoM、脂蛋白的变化。通过建立离体肝灌注模型，检测肝脏ApoM mRNA的变化。结果在尾静脉实验中，瘦素组较生理盐水组血糖增加了14．68％（P〈0．01），胰岛素水平下降了68．82％（P〈0．05），大鼠肝脏ApoM、ApoB和LDL—RmRNA水平分别下降了55．15％（P〈0．05）、48．60％（P〈0．01）及40．43％（P〈0．05），而ApoAI表达增加了149．00％（P〈0．01）。但在离体肝灌注模型中，瘦素对ApoM、ApoAI、ApoB和LDL—R的表达无明显影响（P〉0．05）。结论瘦素对大鼠肝脏脂蛋白和LDL—R表达的影响可能通过其他信使介导，具体机制还需进一步研究。Objective To observe the effects of leptin on the expression of apolipoprotein M＇ s （ApoM） expression in HepG2 cells. Methods A series experiments were performed in the isolated rat＇ s liver perfusion and in normal rats. ApoM protein concentrations in serum were determined by the dot-blotting method and ApoM mRNA levels in liver tissues were determined by real-time reverse transcription-polymerase chain reaction （RT-PCR）. Results After short time leptin administration, serum insulin levels were significantly decreased by 68. 82% （P 〈 0. 05 ）, at the meanwhile blood glucose levels were increased by 14. 68 % （P 〈 0. 01 ） in the leptin treated rats compared to the control rats. Moreover, leptin could significantly decrease hepatic mRNA levels of ApoM,ApoB and LDL-R by 55.15% （P 〈0. 05） ,48.60% （P 〈0. 01 ） and 40.43% （ P 〈 0. 05 ）, respectively, whereas ApoAI mRNA level was increased by 149.00% （P 〈0. 01 ） in the leptin treated rats. However, Leptin could not alter expression of ApoM, ApoAI, ApoB and LDL-R in the isolated liver perfusion （ P 〉 0. 05 ）. Conclusion Leptin influences hepatic expressions of apolipoproteins and LDL-R needs secondary mediator in rats＇ body. The detailed mechanism needs further investigation.

关 键 词：载脂蛋白M 瘦素 载脂蛋白B 低密度脂蛋白受体 

分 类 号：R96[医药卫生—药理学]