复方861对肝星状细胞间质胶原酶及I型胶原基因表达的影响  

Effects of compound 861 on gene expression of interstitial collagenase and type I collagen in HSC- T6 cell

作　　者：王爱民[1] 阴桢宏[2] 马志杰[1] 马红[2] 李洵[1] 王宝恩[2] 

机构地区：[1]解放军252医院消化科,河北保定071000 [2]北京友谊医院肝病研究中心

出　　处：《中华实验外科杂志》2012年第10期1964-1965,共2页Chinese Journal of Experimental Surgery

基　　金：军队“十五”杰出人才基金课题（01J002）;军队“十一五”面上课题（06MA073）

摘　　要：目的观察复方861对HSC．T6细胞间质胶原酶（MMP13）及I型胶原基因表达的影响。方法以不同浓度的复方861（1．00、0．50、0．25g/L）作用于培养的肝星状细胞株HSC—T6细胞48h，收集细胞，提取总RNA；用逆转录一聚合酶链反应（RT—PCR）法测定MMP13及I型胶原的基因表达水平。结果MMP13基因表达水平在复方8611．00、0．50、0．25g／L3组分别为1．03±0．16、0．60±0．08、0．33±0．04，而空白对照组为0．26±0．04。I型胶原基因表达水平在复方8611．00、0．50、0．25g／L3组分别为0．12±0．01、0．34±0．04、0．67±0．10，而空白对照组为3．12±0．46。结论复方861可明显促进HSC—T6细胞间质胶原酶的基因表达，并明显抑制I型胶原的基因表达；且该作用与剂量有关。Objective To investigate the effects of compound 861 on gene expression of interstitial collagenase and type I collagen in HSC-T6 cell. Methods Hepatic stellate cell （ HSC-T6 ） was cultured in medium containing different concentration of compound 861 （1.00, 0. 50, 0. 25 g/L） and cells were collected after incubation for 48 hours. Total RNA of HSC was isolated and gene expression levels of interstitial collagenase （ MMP13 ） and type I collagen were measured by reverse transcription polymerase chain reaction （RT-PCR）. Results MMP13 gene expression level of different groups of compound 861 （ 1.00, 0. 50, 0. 25 g/L） were 1.03 ±0. 16, 0. 60 ±0. 08, 0. 33 ±0.04 respectively and that of control group was 0. 26 ±0. 04. Type I collagen gene expression level of different groups of compound 861 （ 1.00, 0. 50, 0. 25 g/L） were 0. 12 ±0. 01, 0. 34 ± 0. 04, 0. 67 ± 0. 10 respectively and that of control group was 3.12±0. 46. Conclusion Compound 861 may significantly enhance MMP13 gene expression of HSC-T6, but inhibit type I collagen gene expression remarkably and these effects were correlate with the dosage of compound 861.

关 键 词：复方861 HSC—T6细胞 间质胶原酶 I型胶原 基因表达 

分 类 号：R96[医药卫生—药理学]