Wnt1和Wnt8a基因在先天性巨结肠中的表达  

Expression of Wnt1 and WntSa genes in colons of Hirschsprung disease

作　　者：高红[1] 伍美[1] 弭杰[1] 张娟 贾慧敏[2] 王维林[2] 

机构地区：[1]中国医科大学附属盛京医院卫生部小儿先天畸形重点实验室,沈阳110004 [2]中国医科大学附属盛京医院卫生部小儿外科,沈阳110004 [3]辽宁省锦州市妇婴医院小儿外科

出　　处：《中华实验外科杂志》2012年第10期2022-2024,I0002,F0003,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30772277,81170334）

摘　　要：目的观察Wnt1和Wnt8A基因在先天性巨结肠症（HD）中的表达，探讨其在HD发生、发展中的作用。方法对60例HD正常肠段（神经节细胞）和狭窄肠段（无神经节细胞）组织肠管中Wnt1和Wnt8a mRNA和蛋白表达进行定量与比较。结果Wnt1和Wnt8a在HD狭窄段肠管中mRNA表达水平分别为17．66±1．28和29．61±3．23，正常段肠管中mRNA表达水平分别为31．77±2．21和15．01±1．29，差异有统计学意义（P〈0．05）。Westernblot检测结果显示，Wnt1和WntSa在HD狭窄段肠管中蛋白含量为19．51±1．57和33．17±2．09，正常段肠管中蛋白含量分别为37．05±1．23和17．35±0．87，差异有统计学意义（P〈0．05）。免疫组织化学结果显示，Wnt1在正常段肠管中高表达（呈棕黄色），Wnt8a在狭窄段肠管中高表达（呈棕黄色）；Wnt1在狭窄段肠管中低表达（无色或淡黄色），WntSa在正常段肠管低表达（无色或淡黄色）。结论Wnt1和Wnt8amRNA与蛋白在HD的异常表达，提示Wnt1和Wnt8a与HD的发生密切相关，可能在先天性消化道畸形的肠道发育中具有重要作用。Objective To investigate the expression of Wntl and Wnt8a genes in tissue samples from the patients with Hirschsprung disease （HD）. Methods The expression of Wntl and Wnt8a mRNA and proteins were quantitatively analyzed in 60 cases of HD （ aganglionic and ganglionic segments）. Results In the HD patients, there was statistically significant difference in the differential expression of Wntl and Wnt8a mRNA between the stenotic intestine and the normal intestine. The relative expression of Wntl mRNA in the stenotic intestine and normal intestine was 17.66 ± 1.28 and 29. 61 ±3.23 respectively. The relative expression of Wnt8a mRNA in the stenotic intestine and normal intestine was 31.77 ± 2. 21 and 15.01 ± 1.29 respectively （P 〈0. 05）. The protein expressive levels of Wntl and Wnt8a were 19. 51 ±1.57 and 33.17 ±2. 09 in the stenotic intestine, and 37. 05 ± 1.23 and 17.35 ±0. 87 in the normal intes- tine, respectively （ P 〈 0. 05 ） The expression level of Wntl protein was lower in the aganglionic segment than in the ganglionic segment. Immunohstoehemistry revelaed that Wntl was highly expressed in the ganglionic segment, but weakly expressed in the aganglionie segment; WntSa was highly expression in the aganglionic segment, but weakly expressed in the ganglionic segment. Conclusion There is the aberrant expression of Wntl and Wntl mRNA and proteins in HD, suggesting the Wntl and Wnt8a are involved in the pathogenesis of HD, which may play a role in the pathogenesis of congenital malformation of the alimentary tract.

关 键 词：先天性巨结肠症 WNT1 Wnt8a 基因表达 

分 类 号：R726.5[医药卫生—儿科]