丹参注射液腔内注射对早期骨关节炎中一氧化氮合酶的影响  

Effect of intra-articular injection of Salvia on osteoarthritis early by inducible nitric oxide synthase

作　　者：姜婷[1] 姚弘毅[2] 石大玲[1] 程凯[2] 何学斌[2] 

机构地区：[1]华中科技大学同济医学院,武汉430030 [2]华中科技大学同济医学院附属梨园医院

出　　处：《中华实验外科杂志》2012年第10期2060-2061,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察丹参注射液关节腔内注射对膝骨性关节炎早期诱导型一氧化氮合酶（iNOS）的影响。方法将24只雄性大耳白兔随机分为A组（模型组）、B组（阳性对照组），C组（实验组），均用手术方法切开兔膝关节造成膝骨性关节炎（KOA）模型，后行石膏固定术；进而B组腔内注射玻璃酸钠0．3ml／只、c组腔内注射丹参注射液0．3ml／只、A组给予同体积的生理盐水，每周1次，延续4周。4周后处死兔，剥取滑膜、关节软骨检测iNOS含量。结果B组滑膜组织及软骨中iNOS含量分别为（0．83±0．12）gmol／L和（0．13±0．06）μmol／L，与A组比较差异有统计学意义（P〈0．05）；C组滑膜组织及软骨中iNOS含量分别为（0．93±0．15）μmol／L和（0．20±0.08）μmol／L，与A组比较差异有统计学意义（P〈0．05）；但B组滑膜组织及软骨中iNOS含量与C组比较差异无统计学意义（P〉0．05）。结论丹参注射液腔内注射可降低iNOS含量，抑制滑膜炎症，从而促进关节软骨的修复。Objective To observe the effect of Intra-articular Injection of Salvia on knee osteoar- thritis early by inducible nitric oxide synthase （iNOS）, and to explore its therapeutic mechanism. Methods Twenty-four Japanese male white rabbits were made into knee osteoarthritis mode] and randomly divided into 3 groups ： Group A （ model group）, B Group （ Sham group） and Group C （ experimental group）. The rabbits of Group A, B, C were injected normal saline 0. 3 ml, sodium hyaluronate O. 3ml and Salvia 0. 3 ml, respectively. Each rabbits received injection 4 times. Four weeks after the administration, the levels of iNOS in synovial and articular cartilage were measured. Results There were significant differences between the levels of iNOS in synovial and articular cartilage of rabbits in Group A and B, Group A and C （P 〈 0. 05）, and no significant differences between that of rabbits in Group B and C. Conclusion Intra-articular Injection of Salvia could inhibit the expression of iNOS to decrease its level in knee OA, and contribute to slow down the degeneration of articular cartilage.

关 键 词：骨关节炎 丹参注射液 诱导型一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]