抵挡汤早期干预对2型糖尿病大鼠肿瘤坏死因子-α与血管细胞黏附分子-1表达的影响  

Effects of Early Intervention with Didang Decoction on Expression of VCAM-1 and TNF-α in Experimental Type 2 Diabetic Rats

作　　者：常柏[1] 李巧芬[2] 常宝成[1] 潘从清[1] 孟东[1] 陈莉明[1] 李春深[2] 

机构地区：[1]天津医科大学代谢病医院,天津300070 [2]天津中医药大学,天津300193

出　　处：《中国中医药信息杂志》2012年第10期38-40,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30973753)

摘　　要：目的观察抵挡汤早期干预对2型糖尿病大鼠血管病变中大血管的保护作用,探讨其机制。方法采用高脂饲料喂养及链脲佐菌素诱导方法制成大鼠糖尿病模型,抵挡汤早期干预组、抵挡汤中期干预组和抵挡汤晚期干预组分别于实验成模前4周、成模和成模后4周给予抵挡汤灌胃,辛伐他汀组和罗格列酮组大鼠于成模时分别给予辛伐他汀和罗格列酮灌胃,至实验24周时结束。酶联免疫吸附法检测大鼠血清血管细胞黏附分子-1(VCAM-1)水平,实时荧光定量PCR法检测主动脉肿瘤坏死因子-α(TNF-α)mRNA表达。结果与正常对照组比较,模型对照组大鼠血清VCAM-1水平明显升高(P<0.05),主动脉TNF-αmRNA表达明显升高(P<0.05);与模型对照组比较,抵挡汤早期干预组和辛伐他汀组大鼠血清VCAM-1水平明显降低(P<0.05),并且抵挡汤早期干预组、抵挡汤中期干预组和罗格列酮组大鼠主动脉TNF-αmRNA的表达明显降低(P<0.05)。结论抵挡汤早期干预能抑制大鼠主动脉TNF-αmRNA表达升高,抑制大血管病变的炎性损伤,延缓糖尿病大血管病变的发展,发挥其保护大血管的作用。Objective To observe the protective effects of the early intervention with Didang decoction on the experimental type 2 diabetic rats with macrovascular lesions.Methods Experimental diabetic rats were induced by high fat diet and streptozotocin.The rats in Didang decoction late group,Didang decoction middle group and Didang decoction early group were given Didang decoction from 4 weeks before modeling,modeling completed and 4 weeks after modeling respectively to 24th week.The rats in simvastatin group and rosiglitazone group were given simvastatin and rosiglitazone respectively from modeling completed to 24th week.The level of VCAM-1 in serum was detected by ELASA and the mRNA expression of TNF-α in aorta using real-time PCR.Results Compared with normal control group,the level of VCAM-1 in serum and the mRNA expression of TNF-α in aorta were higher in model control group（P0.05）.Compared with model control group,the level of VCAM-1 in serum was lower in Didang decoction early group and simvastatin group（P0.05）,the mRNA expression of TNF-α in aorta was lower in Didang decoction early group,Didang decoction middle group and rosiglitazone group（P0.05）.Conclusion The level of VCAM-1 in serum was reduced and the mRNA expression of TNF-α in aorta of experimental type 2 diabetic rats was inhibited after early intervened with Didang decoction.Thus the inflammatory injury of macrovascular lesions was inhibited,the development of diabetic vascular disease was delayed,and the great vessels was protected.

关 键 词：抵挡汤 糖尿病 大血管病变 肿瘤坏死因子-Α 血管细胞黏附分子-1 大鼠 

分 类 号：R285.5[医药卫生—中药学]