产妇感染乙型肝炎病毒新生儿脐血树突状细胞亚群的变化  

Variations of cord blood dendritic cell subsets isolated from fetuses whose mothers have chronic hepatitis B

作　　者：王秀娟[1] 张剑白[2] 

机构地区：[1]黑龙江省医院儿科,黑龙江哈尔滨150036 [2]哈尔滨医科大学第一临床学院

出　　处：《中国校医》2012年第10期763-764,766,共3页Chinese Journal of School Doctor

基　　金：黑龙江省教育厅资助项目(11511190)

摘　　要：目的观察产妇感染乙型肝炎病毒新生儿脐血中树突状细胞亚群(dendritic,DC)的变化,揭示慢性乙型肝炎宫内感染发生机制,为将来早期阻断儿童慢性乙肝病毒感染提供理论基础。方法采集28名感染乙型肝炎病毒产妇新生儿及12名健康产妇所生新生儿新鲜脐带血,用三色分析法进行免疫荧光标记,24 h内上流式细胞仪进行树突状细胞的检测。结果慢性乙型肝炎病毒感染产妇新生儿脐血中DC1,DC2相对数均高于正常新生儿,但无统计学意义;DC1/DC2与对照组相比差异无统计学意义(P>0.05)。结论慢性乙型肝炎病毒感染产妇新生儿脐血中树突状细胞亚群DC1、DC2相对数较健康产妇新生儿略高,但差异无统计学意义。Objective To understand the variations of cord blood dendritic cell （DC） subsets isolated from fetuses whose mothers have chronic hepatitis B （CHB）, and reveal the transmission mechanism of CHB between mothers and their babies, so as to supply some rationale to lower the CHB morbility of the children and to prevent children from the infection of chronic hepatitis B earlier. Methods A total of 28 cord blood samples from fetuses whose mothers had chronic hepatitis B were detected for the DC subsets, and 12 normal cord blood samples were as controls. All the cord blood samples were marked immediately after being collected, and were detected by flow cytometer in 24 hours. Results The relative quantities of cord blood DC1 and DC2 isolated from the fetuses whose mothers had chronic hepatitis B were higher than those from the fetuses whose mothers were healthy, but there was no significant difference. Conclusions The relative quantities of DC1 and DC2 in the cord blood of the fetuses whose mothers have CHB are both a little higher than those from the fetuses whose mothers were healthy, but there is no significant difference.

关 键 词：树突细胞/免疫学 脑血/细胞学 肝炎 乙型 慢性 产妇卫生保健服务 

分 类 号：R512.62[医药卫生—内科学]