集约化养猪废水SBR中17β-雌二醇高效降解菌的分离鉴定及其降解特性  

Isolation and identification of 17β-estradiol-degrading strains from SBR for swine wastewater and their degradation characteristics

作　　者：方会[1] 陈梅雪[1] 秦德韬[1] 俞峰[1] 强志民[1] 杨敏[1] 

机构地区：[1]中国科学院生态环境研究中心环境水质学国家重点实验室,北京100085

出　　处：《环境工程学报》2012年第10期3361-3366,共6页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金面上项目(20077085);国家高技术研究发展计划(863)项目(2007AA06Z344);国家科技支撑计划(2009BAC57B04)

摘　　要：从集约化养猪废水生物处理SBR的活性污泥中分离到3株高效降解17β-雌二醇(E2)的菌株,分别命名为ha、chs和hc。研究表明,这3株菌以E2为惟一碳源,在4 d内对初始浓度为1 mg/L E2的降解率为70%～95%。25℃条件下菌ha、chs和hc的一级反应动力学常数分别为0.0086、0.072和0.013。在温度为37℃时,3株菌的降解效率最高,在高浓度的氨氮和碱性pH的条件下,这3株菌均存在降解作用。其中,pH 9.05时,一级动力学常数菌ha降至0.0066,菌chs升至0.076,菌hc降至0.012。同时,在添加C源后,对降解有促进作用,并且C/N比在15∶1时降解效果较好。3株菌的一级反应动力学常数分别升到0.027、0.73和0.021。经16S rRNA基因序列分析鉴定为枯草芽孢肝菌(Bacillus subtilis)。Three bacterial strains,named ha,chs,hc,of high efficiently degrading 17β-estradiol（E2） were isolated from the active sludge of a SBR for swine wastewater treatment.It was identified that,the degradation rate of the three strains reached 70%～95% under the E2 concentration of 1 mg/L as the sole carbon source within 4 days,and the constants of first order reaction kinetics of strain ha,chs,hc were 0.0086,0.072,0.013 at 25℃,respectively.It was found that the degradation efficiency of the 3 strains were maximized at 37℃,and the degradation ability could be kept on the high concentration of ammonia and alkaline conditions.When pH was 9.05,the constants of both strain ha and strain hc dropped to 0.0066 and 0.012,while strain chs increased to 0.076.The constants of first order reaction kinetics of the 3 strains increased to 0.027,0.73 and 0.021,when the ratio of C/N was 15∶ 1.It implied that adding carbon source would promote the degradation process.According to the analysis of 16S rRNA gene sequences,the 3 strains were identified as Bacillus subtilis.

关 键 词：养猪废水 17Β-雌二醇 生物降解 枯草芽孢肝菌 

分 类 号：X703[环境科学与工程—环境工程]