花生体细胞胚发生发育过程中淀粉代谢的动态变化  

Study on Dynamics of Starch Metabolic Process during Somatic Embryogenesis of Peanut ( Arachis hypogaea L.)

作　　者：林荣双[1] 王庆华[1] 梁丽琨[1] 郑秋生[1] 肖显华[1] 

机构地区：[1]烟台大学生物化学系,山东烟台264635

出　　处：《烟台大学学报（自然科学与工程版）》2000年第3期226-229,共4页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目! (Y97D16 0 75 )

摘　　要：将花生实生苗的幼对接种于MS固体诱导培养基上诱导体细胞胚发生 ,用PAS染色法观察了体细胞胚发生发育过程中的淀粉代谢的动态变化 .结果表明 ,在体细胞胚的发育过程中 ,淀粉表现出两次积累高峰 ,第一次在胚性细胞团中 ,淀粉粒大而密集 ;随着胚性细胞的分裂 ,淀粉逐渐被消耗 ,到多细胞原胚期胚体内淀粉趋于消失 .球形胚期 ,淀粉粒又开始积累 ,出现第二次积累高峰 ;到成熟胚期 。Somatic embryos are induced from young leaflets of peanut( Arachis hypogaea L. )seedlings on MS induced medium. PAS reaction reveals the dynamic change of starch metabolism during somatic embry ogenesis. There is a peal accumulation of starch in the form of big and crowded grains in the embryogenic cells, but they gradually diminish with the division of the cells and disappear completely when multicellular proembryos are formed. The other accumulation of starch grains appears in the globular stage and again they decrease gradually. In the mature embryos, starch accumulate only in the cells of growing point. Both of the peak accumulations of starch grains supply material and energy for the development of embryos.

关 键 词：花生 体细胞胚 发生 发育 淀粉代谢 

分 类 号：S565.201[农业科学—作物学] Q81[生物学—生物工程]