超滤离心法测定连翘酯苷脂质体包封率  

Determination of entrapment efficiency of forsythoside liposome by ultrafiltration

作　　者：庄英华[1] 张中文[1] 韩伟[1] 王惠川[1] 吴国娟[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国新药杂志》2012年第18期2209-2211,2216,共4页Chinese Journal of New Drugs

基　　金：国家自然科学基金(31172362;30972215);"十二五"支撑计划(2011BAD34B01-07);北京市教委专项(KM 201010020008);北京市属高校人才强教深化计划(PHR2009-07135)

摘　　要：目的:建立连翘酯苷脂质体包封率的测定方法。方法:通过逆相蒸发法制备连翘酯苷脂质体,运用超滤离心法测定其包封率。结果:超滤离心法能快速有效的对脂质体与游离药物进行分离,游离药物平均回收率在98.75%～101.71%之间,超滤加样回收率在95.67%～98.76%之间;此法测定3批样品,所得包封率为72.77%～74.07%,RSD为0.56%～1.77%。结论:超滤离心法操作简单、快速,可用于连翘酯苷脂质体包封率的测定。Objective： To establish a method for determining entrapment efficiency of forsythoside liposomes. Methods： Forsythoside liposomes were prepared by a reverse evaporation method, and then the entrapment efficiency was determined by ultrafihration. Results： The free drug was separated from the forsythoside liposomes efficiently. Average recovery rate of free forsythoside was 98.75% - 101.71% , and the recovery rate was 95.67% - 98.76% in the process of ultrafihration. The entrapment efficiency of three lot samples was 72.77% - 74.07% with RSD of 0.56% -1.77% by ultrafihration. Conclusion： The ultrafiltration method can simply and immediately measure entrapment efficiency of forsythoside liposomes after preparation.

关 键 词：连翘酯苷 脂质体 包封率 高效液相色谱法 超滤离心 

分 类 号：R943[医药卫生—药剂学]