β-细辛醚通过PKA/CREB信号通路对快速老化痴呆小鼠脑组织凋亡机制的影响  

Effect of β-asarone on the PKA/CREB signaling pathway in the rapid aging dementia mice

作　　者：王睿[1] 费洪新[2] 李晓明[1] 王伟[2] 刘韩[1] 牛英才[1] 刘向民[2] 周涛[2] 

机构地区：[1]齐齐哈尔医学院医药科学研究所,黑龙江齐齐哈尔161006 [2]齐齐哈尔医学院基础医学院,黑龙江齐齐哈尔161006

出　　处：《中国医药导报》2012年第28期30-32,共3页China Medical Herald

基　　金：黑龙江省自然科学基金面上项目(D200955)

摘　　要：目的研究β-细辛醚对快速老化痴呆小鼠大脑组织蛋白激酶A(PKA)、cAMP反应元件结合蛋白(CREB)的影响。方法选取健康昆明小鼠10只作为对照组,30只快速老化小鼠被随机分为模型组(10只)、实验组(10只)、治疗组(10只)。对照组采用生理盐水灌胃,2 mL/次;实验组采用β-细辛醚混浊液灌胃,2 mL/次;治疗组采用脑复康水溶液5 mg/mL灌胃,2 mL/次;模型组采用生理盐水灌胃,2 mL/次。所有小鼠均每天灌胃1次,持续3周。3周后进行小鼠行为学测试,检测脑组织PKA、CREB酶改变及光镜和电镜下神经元细胞形态变化。结果实验组和治疗组与模型组相比,潜伏期明显缩短、在原平台停留时间延长,PKA、CREB活性与模型组比较均明显增高,差异有统计学意义(P<0.05)。光镜下观察实验组海马神经元损伤较轻,大部分细胞核清楚完整,电镜下核周隙清晰,细胞器清晰,神经纤维较丰富。结论β-细辛醚能提高快速老化痴呆小鼠知识记忆能力,影响PKA/CREB传导路径和突触结构,减少快速老化痴呆小鼠大脑组织神经元损伤,对快速老化痴呆小鼠有明显治疗作用。Objective To study the effect of β-asarone on the PKA/CREB in the brain of the rapid aging dementia mice. Methods 10 healthy Kunming mice were selected as control group, 30 rapid aging mice were divided into experiment group （10）, treatment group （10）, model group （10）. Mice in the control group were lavaged with physiological saline, 2 mL/time; mice in the experiment group were lavaged with 13-asarone, 2 mL/time; mice in the treatment group were lavaged with Piracetam Solution （5 mg/mL）, 2 mL/time; mice in the model group were lavaged with physiological saline, 2 mL/time. And continued with 3 weeks. After 3 weeks （1 time/d）, the mice behavior in every group were observed, PKA, CREB enzyme changes in the brain and the changes of neuron cells in the light microscope and electron icroscope were detected. Results The experiment group and the treatment group showed significantly shorter incubation period, longer or iginal platform compared with the model group, PKA, CREB activity was significantly increased compared with the model group, the dif- ference was statistically significant （P 〈 0. 05）. For the experiment group, under light microscope, the damage of neurons was ligher, most of the cell nucleus were clearly and completely, under electron microscope, perinuclear space was clearly visible, most of the organelles were visible, nerve fibers were abundant. Conclusion β-asarone can enhance the rapid ag- ing dementia mice learning and memory ability, improve the PKA/CREB signaling pathway and affect synaptic structure, also can reduce neuron cells damage in the brain. It has significant therapeutic effect for rapid aging dementia mice.

关 键 词：Β-细辛醚 CAMP反应元件结合蛋白 神经元 

分 类 号：R332[医药卫生—人体生理学]