甘蓝种传镰刀菌的鉴定及其致病性研究  

Study on Identification of Cabbage Seedborne Fusarium Species and Their Pathogenicity

作　　者：刘梅[1] 王丽君[1] 曹莎[1] 吴学宏[1] 黄俊生[2] 

机构地区：[1]中国农业大学植物病理学系/农业部植物病理学重点开放实验室,北京100193 [2]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《种子》2012年第9期26-29,33,共5页Seed

基　　金：公益性行业(农业)科研专项经费项目资助(编号:200903049)

摘　　要：针对春甘45和红亩紫甘蓝种子上分离得到的两株镰刀菌(Fusarium-CG和Fusarium-HM),结合形态学特征及rDNA ITS序列分析进行了鉴定,并研究了其对甘蓝种子发芽的影响和幼苗的致病性。结果表明:2株镰刀菌均为尖孢镰刀菌(Fusarium oxysporum);对甘蓝种子发芽有一定的影响,发芽势、发芽率、发芽指数、活力指数等指标均降低,其中106个/mL孢子悬浮液处理后,活力指数显著降低;能导致甘蓝幼苗出现典型的枯萎症状,柯赫氏法则检验证明其具有致病性。甘蓝种子传带尖孢镰刀菌并具有致病性为国内首次报道。Fusarium-CG and Fusarium-HM, were isolated from cabbage seeds （cvs. Chungan No. 45 and Hongmuziganlan）. The two isolates were identified using morphological methods and rDNA ITS sequencing analysis. Effects on seed germination and pathogenicity of them were also determined. The results showed that the two Fusarium strains were Fusarium oxysporum. They had significant effect on seed germination, compared to control, which causing reduction of germination energy, percentage germination, germination index and vigor index,and vigor index decreased significantly when treated by 106spores/mL spore suspension; they were pathogenic to seedlings and caused typical wilt symptom on seedlings, fulfilling Koch＇ s postulates. It＇ s the first report of cabbage seedborne F. oxysporum can be pathogenic to cabbage seedlings in China.

关 键 词：甘蓝 种传镰刀菌 种子发芽 病原性 

分 类 号：S635[农业科学—蔬菜学]