减肥类保健食品中违禁添加酚酞、西布曲明及其两种衍生物的检测  

Identification of Phenolphthalein,Sibutramine and Its Two Derivatives Added Illegally in Slimming Health Care Food

作　　者：邱颖姮[1] 邬晓鸥[1] 冯丰凑[1] 庞学斌[1] 李军[1] 

机构地区：[1]深圳市药品检验所,广东深圳518057

出　　处：《中国医药工业杂志》2012年第10期861-864,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了高效液相色谱-二极管阵列检测器法测定减肥类保健食品中违禁添加的酚酞、西布曲明及其两种衍生物。采用C18色谱柱,以0.02 mol/L乙酸铵溶液(用乙酸调至pH 4.0)-乙腈(56︰44)为流动相,检测波长225 nm。酚酞、西布曲明及其两种衍生物在0.01～0.2 mg/ml范围内线性关系良好,回收率为96.8%～103.0%,RSD小于2.3%。采用液相色谱-串联质谱法验证了阳性结果。An HPLC-DAD method was established for the determination of phenolphthalein, sibutramine and its two derivatives added illegally in slimming health care food. A C18 column was used with the mobile phase of 0.02 mol/L ammonium acetate solution （adjusted to pH 4.0 by acetic acid）-acetonitrile （56 ： 44） at the detection wavelength of 225 nm. The calibration curves of phenolphthalein, sibutramine and its two derivatives were linear in the range of 0.01 - 0.2 mg/ml. The recoveries were 96.8 % - 103.0%, with RSDs less than 2.3 %. The positive results were identified by LCMS/MS.

关 键 词：酚酞 西布曲明 高效液相色谱 液相色谱-串联质谱 检测 

分 类 号：TQ460.7[化学工程—制药化工] O657.7[理学—分析化学]