透骨草杀虫活性成分分离鉴定及其生物活性研究  

Isolation,structure identification and bioactivity of active ingredients from Phryma leptostachya

作　　者：肖新敏[1] 胡兆农[1] 姬志勤[1] 师宝君[1] 张继文[2] 魏少鹏[1] 吴文君[1] 

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《农药学学报》2012年第5期583-586,共4页Chinese Journal of Pesticide Science

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB126105);国家公益性行业(农业)科研专项(200903052);国家自然科学基金(31171868;31071704)

摘　　要：采用柱层析分离、高效液相色谱切分和生物活性追踪法,从透骨草Phryma leptostachya L.石油醚提取物中分离出2个具有杀虫活性的化合物(B1和B2),采用质谱、核磁共振氢谱和碳谱,并结合相关文献对其结构进行鉴定,发现其分别为双氧木脂素A和E。首次以粘虫Mythimnaseparata、槐尺蠖Semiothisa cinerearia、小菜蛾Plutella xylostella、小地老虎Agrotis ypsilon和家蝇Musca domestica为试虫,测定了这2个化合物的杀虫活性。结果表明:化合物B1和B2对3龄粘虫24 h的胃毒致死中浓度(LC50值)分别为34.9和52.8μg/mL,24 h触杀致死中量(LD50值)分别为0.09和0.26μg/头;对3龄槐尺蠖24 h的胃毒LC50值分别为66.1和1 675μg/mL,触杀LD50值分别为0.049和1.33μg/头;对家蝇成虫24 h的胃毒LC50值分别为39.2和969μg/mL,触杀LD50值分别为0.047和1.12μg/头。Two compounds,haedoxane A and E were isolated from the petroleum ether extract of the roots and stems of Phryma leptostachya L. by silica gel chromatograph, high pressure liquid chromatograph（HPLC） and bioassay-guided fractionation. The bioassay results showed that haedoxane A and E had stomach toxic activity with the LCs0 value of 24 h 34.9 and 52.8 μg/mL against 3rd instar larvae of Mythimna separata, against 3rd instar larvae of Semiothisa cinerearia with the LCs0 value of 24 h 66. 1 and 1 675 μg/mL, and against Musca domestica with the LC50 value of 24 h 39.2 and 969 μg/mL. Haedoxane A and E had topical toxic activity with the LD50 value of 0. 09 and

关 键 词：透骨草 杀虫活性 双氧木脂素A 双氧木脂素E 

分 类 号：Q965[生物学—昆虫学] S482.39[农业科学—农药学]