荧光金纳米团簇的制备及其在铜离子检测中的应用  

Preparation of Fluorescent Gold Nanoclusters and Its Application to the Determination of Copper Ion

作　　者：张浩琪[1] 梁国熙[1] 吴向阳[1] 潘宏程[2] 

机构地区：[1]江苏大学环境学院,江苏镇江212013 [2]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《分析科学学报》2012年第5期608-612,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21005033);江苏省自然科学基金(No.BK2011242);广西自治区自然科学基金(No.0991082);江苏省博士后科研资助计划(1002010B);江苏大学高级专业人才科研启动基金(10JDG038)

摘　　要：在水溶液中以谷胱甘肽(Glutathione,GSH)为稳定剂和还原剂,制备了具有较好荧光性能的金纳米团簇(GSH-AuNCs),对其结构和荧光性能等进行了表征。基于Cu2+对该GSH-AuNCs的荧光具有选择性猝灭作用建立了一种快速且简便的检测痕量Cu2+的方法。考察了检测体系中GSH-AuNCs的浓度、反应时间、pH值等因素对测定的影响。结果表明,在最优实验条件下,GSH-AuNCs的荧光强度与Cu2+的浓度分别在5.0×10-9～4.0×10-6 mol/L(R=0.9940),4.0×10-6～2.0×10-5 mol/L(R=0.9950)范围呈良好线性关系,检出限(S/N=3)为2.0×10-9 mol/L。该方法成功地应用于实际水样中Cu2+的检测。The fluorescent gold nanoclusters that capped with glutathione were applied for ultra-sensitive Cu2＋ sensing. The sensing approach was based on the fluorescence of the GSH-AuNCs selectively quenched in the presence of Cu2＋. Experimental results showed a low interference response towards other metal ions. The possible quenching mechanism was discussed on the basis of the binding between glutathione and the Cu2＋. The response of the optical sensor was linearly proportional to the concentration of Cu2＋ ranging from 5.0×10^-9 to 4.0×10 ^-6 mol/L and 4.0×10^-6 to 2.0×10^-5 mol/L, and the limit of detection（LOD） for Cu2＋ at a signal to noise ratio of 3 was 2. 0 ×10^-9 mol/L. The developed method has been successfully applied to the detection of Cu2＋ in tap-water and lake-water samples.

关 键 词：金纳米团簇(GSH—AuNCs) 荧光猝灭 铜离子 

分 类 号：O657.3[理学—分析化学]