高效液相色谱法测定细胞内外齐墩果酸含量  

Determination of Intracellular and Extracellular Oleanolic Acid by High Performance Liquid Chromatography

作　　者：王均[1] 朱链链[2] 陈志琼[1] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]乐山职业技术学院,四川乐山614000

出　　处：《分析科学学报》2012年第5期737-740,共4页Journal of Analytical Science

摘　　要：本文建立了高效液相色谱法快速准确测定A549细胞内外齐墩果酸(OA)的含量。该法采用Hypersil ODS色谱柱(250×4.6mm,5μm);流动相为pH=3.38的甲醇-水(体积比为90∶10),流速1.0mL/min,柱温30℃,检测波长210nm,进样量为20μL。结果表明,细胞内液中OA在1.020～20.40μg/mL范围内线性关系良好(r=0.9996),平均回收率为97.57%;细胞外液中OA在5.100～81.60μg/mL范围内线性关系良好(r=0.9998),平均回收率为97.37%。该方法简便易行,准确度高,重现性好,可用于A549细胞内外OA浓度的动态变化规律研究。A high performance liquid chromatography method for the determination of intracellular and extracellular oleanolic acid（OA） was developed in this paper. The chromatographic conditions were as follow, stationary phase,ODS（250×4.6 ram, 5μm） . mobile phase 90 ： 10 （V/V） methanol-water （pH - 3.38）;flow rate 1.0 mL/min;column temperature 30 ℃ ;detection wavelength 210 nm and the injection volume 20 μL. The results indicated that the linear range of intracellular OA was 1. 020-20.40 μg/mL （r=0. 9996）, the average recovery was 97.57%;and the linear range of extracellular OA was 5. 100- 81.60 μg/mL（r= 0. 9998）, the average recovery was 97. 37%. The method was simple, accurate and precise,which can be used for the dynamic study of intracellular and extracellular OA.

关 键 词：高效液相色谱 细胞内外 齐墩果酸 

分 类 号：O657.72[理学—分析化学]