健康屠宰猪中博卡病毒的检测及遗传演化分析  

Detection and genetic analysis of bocavirus in healthy swine

作　　者：张志[1] 王赛赛[2] 李岚[3] 刘爽[1] 李蕾[4] 郑辉[1] 吴发兴[1] 张燕霞[1] 李晓成[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032 [2]福建农林大学,福建福州350002 [3]云南农业大学,云南昆明650201 [4]东北农业大学,黑龙江哈尔滨150030

出　　处：《畜牧与兽医》2012年第10期9-12,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家支撑计划项目(2007BAD86B05)

摘　　要：为了解我国健康猪群中猪博卡病毒(PBoV)的感染状况,从屠宰场内采集猪淋巴结、脾脏和扁桃体等组织,分别采用套式PCR进行猪博卡病毒1型和2型的检测。结果表明,28份样品中有2份样品用猪博卡病毒2型引物可扩增出439 bp的特异性条带;结构蛋白VP1基因序列分析表明,这2份样品均为猪博卡病毒2型感染,且其VP1序列与猪博卡病毒参考株HM053694的同源性最高,分别达93.8%和93.3%,同属PBoV1/PBoV2进化分支;而与另一个猪博卡病毒V6/V7进化分支的同源性仅为35.1%～39.2%,距离较远。Lymphoid organ, spleen and tonsil from pigs in a slaughter farm were collected for detection of bocavirus by nested RT-PCR. The result showed that two were infected with porcine bocavirus type 2 （PBoV2） in 28 samples. The VP1 sequence analysis indicated that the homologies of these two strains were 93.8% and 93.3% with porcine bocavirus strain HM053694 in V1/V2 cluster, but 35.1% - 39. 2% with V6/V7 cluster, which indicates a close relationship with PBoV1/PBoV2 subgroup.

关 键 词：猪博卡病毒 套式PCR 遗传演化 健康猪 

分 类 号：S852.6[农业科学—基础兽医学]