新型抗氧化肽SS31体外辐射防护作用及其机制的初步研究  

An in vitro preliminary study of the radio-protective properties of antioxidant peptide SS31

作　　者：邹佳[1] 杨伟志[2] 王清清[1] 付洁[1] 董立厚[1] 向慎思[1] 高新[1] 孙云娟[1] 宋海峰[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所药理毒理研究室,北京100850 [2]同济大学附属上海第十人民医院骨科,上海200072

出　　处：《辐射研究与辐射工艺学报》2012年第5期291-296,共6页Journal of Radiation Research and Radiation Processing

基　　金："十一五"国家科技重大专项"重大新药创制"项目(2009ZXJ09001-028)资助

摘　　要：为探讨新型抗氧化剂SS31在体外对细胞的辐射防护作用和初步机制,用60Coγ射线照射细胞,用不同浓度的SS31对细胞进行干预,观察照射后细胞的增殖、生存状态、细胞内ROS水平和线粒体膜电位的改变,并将药物干预组和阴性对照组进行比较。结果发现:7.5 Gy的γ射线照射后24–72 h,细胞增殖活性显著降低;48 h内细胞凋亡和坏死比率显著增加,并伴随胞内ROS增长、线粒体膜电位下降和细胞周期阻滞。SS31能明显改善这些反应,药物干预组与阴性对照相比有统计学差异。通过本实验,观察到了SS31具有明显的体外辐射防护作用,提示其有潜力作为一种新型的辐射防护剂。In order to research the radio-protective properties of a new antioxidant peptide, SS31 in vitro, cells were irradiated by 60Co γ-rays and incubated with SS31 in different concentration. Cell viability, mitochondrial mem- brane potential, cell living rate and ROS generation were investigated and compared with that of control group. After irradiation with 7.5 Gy γ-rays, SS31 could reduce the cell injury significantly while cell viability decreased and apoptotic rate increased definitely, with higher ROS generation, mitochondrial membrane potential collapsing and cell cycle arrest if without intervention by use of SS31. Compared with the control group, SS31 intervention group showed statistical difference. This study has been revealed that SS31 has potency to reduce acute radiation injury and thereby act as a new radio-protective agent.

关 键 词：SS31 抗氧化 辐射防护 

分 类 号：Q691[生物学—生物物理学] R961[医药卫生—药理学]