谷胱甘肽乙酯对烟雾吸入性肺损伤的作用  

Effects of glutathione ethyl ester on smoke inhalation lung injury

作　　者：张曙[1] 齐菲[1] 左振华[1] 刘伟[2] 汪建新[1] 

机构地区：[1]100853北京,解放军总医院呼吸内科 [2]710038陕西西安,第四军医大学唐都医院呼吸内科

出　　处：《中国危重病急救医学》2012年第10期624-627,共4页Chinese Critical Care Medicine

基　　金：解放军总医院创新基金课题（07CX304）

摘　　要：目的探讨合成谷胱甘肽（GSH）的前体谷胱甘肽乙酯（GSEt）对大鼠烟雾吸入性肺损伤的影响。方法按随机数字表法将60只雄性SD大鼠均分为正常组、模型组及GSEt低、高剂量组。建立烟雾吸入性肺损伤模型，GSEt治疗组分别于烟雾吸入后5min腹腔注射GSEt 50mg/kg和150mg／kg。各组分别于伤后2、12、24h取腹主动脉血进行血气分析；处死大鼠取肺，测定支气管肺泡灌洗液（BALF）中GSH活性；检测肺组织匀浆上清液中GSH、过氧化氢酶（CAT）及谷胱甘肽还原酶（GR）活性；制作肺组织病理切片，光镜下观察病理变化。结果与正常组比较，模型组伤后各时间点动脉血氧分压（PaO2）、BALF中GSH活性及肺组织GSH、GR、CAT活性均显著下降。与模型组比较，GSEt高剂量组伤后12hPaO2（mmHg，1mmHg=0．133kPa）显著升高（82．9±7．0比63．9±6．5，P〈0．05），伤后12h、24h肺组织GSH活性（mg／g）显著升高（12h：2．19±0．41比0．79±0．21，24h：1．75±0．47比0．67±0．10，均P〈0．05）；GSEt低剂量组伤后24h和GSEt高剂量组伤后12h、24h肺组织CAT活性（u/g）显著升高（低剂量组24h：70．1±5．5比56．3±5．0；高剂量组12h：90．9±8．1比67．9±6．1，24h：94．7±7．7比56．3±5．0，均P〈0．05）；GSEt高剂量组伤后24h肺组织GR活性（mmol／g）显著升高（5．25±0．77比4．37±0．64，P〈0．05）。光镜下观察伤后12hGSEt高剂量组较模型组肺组织损伤程度明显减轻，炎性渗出减少，无明显出血。结论GSEt治疗能够提高烟雾吸人性肺损伤肺组织抗氧化能力，对肺脏具有较好的保护作用。Objective To investigate the effects of glutathione （GSH） precursor glutathione ethyl ester （GSEt） on smoke inhalation induced lung injury rats. Methods Sixty healthy male Sprague-Dawley （SD） rats were divided into groups by random digits table method, which included noiTnal group, model group, GSEt high dose group and GSEt low dose group. Smoke inhalation induced lung injury rats model was established. GSEt treatments were given through intraperitoneal injection for 50 mg/kg or 150 mg/kg 5 minntes after the injury. Arterial blood gas analysis was monitored at 2, 12 and 24 hours after injury in each group. Rats were sacrificed for lungs, and bronchoalveolar lavage fluid （BALF） was collected for analysis of GSH activity; and the activity of GSH, catalase （CAT） and glutathione reductase （GR） were detected in pulmonary tissue homogenate. The changes of pulmonary tissue pathology was observed through light microscope. Results Compared to normal group, arterial partial pressure of oxygen （PaO2） in model group were decreased significantly in each time ; the activity of GSH in BALF, and the activity of GSH, CAT, GR in lung tissue were also observed decreased significantly. Compared with model group, GSEt treatment （150 mg/kg） with the PaO2 （mm Hg, 1 mm Hg=0.133 kPa）advanced at 12 hours （82.9 ± 7.0 vs. 63.9 ± 6.5, P〈0.05 ）, the activity of GSH （rag/g） was increased at the 12 hours and 24 hours （ 12 hours： 2.19 ±0.41 vs. 0.79 ± 0.21, 24 hours： 1.75 ± 0.47 vs. 0.67 ± 0.10, both P〈0.05 ）; the activity of CAT （U/g） in GSEt low dose group （50 mg/kg） was increased at the 24 hours and the same increase was also observed in GSEt high dose group （ 150 mg/kg） at 12 hours and 24 hours （low dose group 24 hours： 70.1 ± 5.5 vs. 56.3 ± 5.0; high dose group 12 hours： 90.9± 8.1 vs. 67.9 ± 6.1, 24 hours： 94.7± 7.7 vs. 56.3 ± 5.0, all P〈0.05） ; the activity ofGR （mmol/g） in GSEt high dose group was increased at 24 hours （5.25 ± 0.77 vs. 4.37

关 键 词：谷胱甘肽乙酯 烟雾吸入 肺损伤 氧化应激损伤 

分 类 号：R563.8[医药卫生—呼吸系统]