悬浮红细胞储存时间对制备的洗涤红细胞钾离子和游离血红蛋白含量的影响  

Influence of the storage time of red blood cells on the concentrations of and FHb of the prepared washed red blood cells

作　　者：冯博[1] 段澜[1] 王景文[1] 任芙蓉[1] 

机构地区：[1]北京市红十字血液中心,北京100088

出　　处：《中国输血杂志》2012年第9期834-836,共3页Chinese Journal of Blood Transfusion

基　　金：卫生部卫生行业科研专项(201002005)

摘　　要：目的了解不同储存时间的悬浮红细胞(悬红)制备的洗涤红细胞中钾离子浓度([K+])和游离血红蛋白含量(FHb)。方法将储存3 d(3 d组,n=6)、16 d(16 d组,n=6)及31～35 d(31～35 d组,n=7)的悬红,手工制备成洗涤红细胞,分别于制备前、制备后0、16和24 h取样测定其[K+]和FHb。结果 3 d组、16 d组和31～35d组悬红制备的洗涤红细胞24 h时[K+]分别为(3.26±0.57)、(3.39±1.12)及(2.97±0.95)mmol/L(P>0.05),且均低于人体正常[K+]上限;3组制备的洗涤红细胞24 h时的FHb分别为(0.29±0.18)、(0.63±0.40)及(1.06±0.55)g/L,其中31～35 d组制备的洗涤红细胞的FHb明显高于3 d组(P<0.01)。结论悬红储存时间对制备的洗涤红细胞的[K+]影响不大,制备的洗涤红细胞24 h保存期内的[K+]均低于人体正常参考值上限,但对FHb含量有较大影响。建议尽量避免使用接近储存期末的悬红制备洗涤红细胞。Objective To study the influence of the storage time of red blood cells （RBCs） on the concentrations of potassium （ [ K ＋ ] ） and free hemoglobin （FHb） of prepared washed red blood cells （ washed RBCs）. Methods RBCs preserved for 3 d （3 d group,n =6） ,16 d （16 d group,n =6） and 31 -35 d （31 35 d greup,n =7） were chosen to pre- pare washed RBCs manually,and samples were taken at the time before preparation,0,16 and 24 h after preparation, re- spectively. [K＋ ] and FHb were measured. Results The [K~ ] of washed RBCs 24 h after preparation of 3 d group,16 d group and 31 ~35 d group were （3.26 ~0. 57）, （3.39 ~ 1.12） and （2.97 ~0. 95） retooL/L, respectively （P 〉0. 05）, and all the [ K ＋ ] were still lower than the upper limit of [ K ＋ ] in normal human bodies. The FHb of washed RBCs 24 h af- ter preparation of these three groups were （0. 29 ~ 0. 18）, （0. 63 ~ 0. 40） and （ 1.06 ~ 0. 55 ） g/L, respectively. The FHb of washed RBCs 24 h after preparation of 31 ~ 35 d group was significantly higher than that of 3 d group （P 〈 0. 01 ） ~ Conclusion There is little influence of the storage time of RBCs on the [ K ＋ ] of prepared washed RBCs, with the [ K ＋ ] of all prepared washed RBCs lower than the upper limit of [ K＋] in normal human bodies. However,the storage time of RBCs affects the FHb of prepared washed RBCs significantly, so RBCs close to the end of storage should be avoided to pre- pare washed RBCs.

关 键 词：悬浮红细胞 储存时间 洗涤红细胞 钾离子浓度 游离血红蛋白 

分 类 号：R446.112[医药卫生—诊断学] R457.12[医药卫生—临床医学]