导痰汤对颈动脉硬化家兔内皮细胞间黏附分子-1及p38表达的影响  

Influences of Daotan Tang on expressions of ICAM-1 and p38 in rabbits with carotid atherosclerosis

作　　者：黄小波[1] 李宗信[1] 陈文强[1] 王宁群[1] 吴犀翎[1] 

机构地区：[1]首都医科大学宣武医院中医科,北京100053

出　　处：《北京中医药大学学报》2012年第9期620-622,637,I0002,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30772843;30801481);北京市科技新星项目No.2008A090);北京市中医药科技项目(No.JJ 2009-037)

摘　　要：目的研究导痰汤是否能够通过对内皮细胞间黏附分子-1(ICAM-1)和p38通路的调节而干预颈动脉硬化。方法新西兰白兔随机分为对照组、模型组和导痰汤大、中、小剂量组(10、8、6 g/kg导痰汤灌胃)。通过免疫组化方法,观察导痰汤对颈动脉硬化家兔血管内皮细胞ICAM-1和p38表达的影响。结果模型组血管壁内皮细胞的胞浆中有ICAM-1和p38强阳性表达,显著高于对照组(P<0.01)。导痰汤治疗组细胞内ICAM-1和p38阳性表达显著低于模型组(P<0.01),且随剂量的增加,导痰汤对ICAM-1和p38的抑制逐渐增强;而p38活性与ICAM-1水平呈显著正相关(r=0.757,P<0.01)。结论导痰汤可以有效抑制颈动脉硬化家兔血管内皮细胞中ICAM-1和p38的表达。Objective To study whether Daotan Tang can interfere carotid atherosclerosis through regulating intercellular adhesion molecular-1 （ICAM-1） and p38 pathway. Methods New Zealand rabbitswere randomly divided into control group, model group and treatment groups with Daotan Tang in different doses [ high-dose group （10 mL/kg）, mid-dose group （8 mL/kg） and low-dose group （6 mL/ kg） given intragastrically]. The influences of Daotan Tang on the expressions of ICAM-1 and p38 were observed by using immunohistochemistry technique. Results In model group, the expressions of ICAM- 1 and p38 showed strong positive in endochylema of endothelial cells of vessel wall, which were significantly higher than those in control group （P 〈0.01 ）. The positive expressions of ICAM-1 and p38 were significantly lower in treatment groups than those in model group （ P 〈 0. 01 ）, and this inhibitory effect increased as the dose of Daotan Tang increased. The activity of p38 was significantly correlated to ICAM-1 level （ r = 0. 757, P 〈 0.01 ）. Conclusion Daotan Tang can effectively inhibit the expressions of ICAM-1 and p38 in vessel endothelial cells in rabbits with carotid atherosclerosis.

关 键 词：导痰汤 颈动脉硬化 细胞间黏附分子-1 P38 兔 

分 类 号：R285.5[医药卫生—中药学]