银杏叶提取物对急性肝衰竭大鼠FADD表达的影响  

Effects of ginkgo biloba extract on liver FADD in acute liver failure

作　　者：王树芳[1] 徐海英[2] 李萌[1] 康静[1] 许重杰[1] 李永海[1] 

机构地区：[1]新乡医学院生命科学技术系,河南新乡453003 [2]黄河科技学院微生物教研室,河南郑州450063

出　　处：《中国现代医学杂志》2012年第22期23-26,共4页China Journal of Modern Medicine

基　　金：河南省教育厅自然科学研究计划项目(No:2010A350002);新乡医学院高学历人才资助项目;医学细胞生物学省重点实验室资助

摘　　要：目的观察银杏叶提取物(ginkgo biloba extract,GBE)对硫代乙酰胺(TAA)损伤的大鼠肝脏的作用及对FADD的影响,并探讨其作用机制。方法实验分5组,GBE低、中、高剂量组(50、100、200 mg/kg)连续灌服GBE 30 d,1次/d,正常对照组和模型组分别给与等体积的生理盐水;模型组、GBE低、中、高剂量组第28 d用TAA(300 mg/kg)腹腔注射,正常对照组腹腔注射等体积的生理盐水。用试剂盒检测血清中AST、ALT的水平,并分别用RT-PCR和Western blot的方法检测各组肝脏FADD在mRNA和蛋白水平的表达情况。结果模型组与正常组相比,血清中ALT、AST水平明显升高,肝组织中FADD在mRNA和蛋白水平均上调;而GBE组与模型组相比,血清中ALT、AST水平明显降低,肝组织中FADD在mRNA和蛋白水平均下调。结论肝衰竭过程中FADD参与肝细胞的凋亡,GBE可通过FADD抑制急性肝衰竭大鼠肝细胞凋亡,从而对肝脏起到保护作用。【Objective】To study the protection effect of ginkgo biloba extract（GBE） on TAA-induced acute liver failure（ALF）.【Methods】46 rats were divided into five groups：The control group,the model group and GBE group.The model mice of acute liver failure were set up by giving intraperitoneal injection of TAA（300 mg/kg）,the serum ALT and AST were determined,FADD were analyzed with RT-PCR.The protein expressions of FADD were analyzed with Western blot.【Results】The serum ALT and AST in mice in model group were increased significantly compared with control group.The serum ALT and AST in mice in GBE group were decreased significantly compared with model group.Compared with model group,the gene and protein expression of FADD was significantly up regulated in liver in GBE group.【Conclusion】GBE could protect hepatocytes from apoptosis and necrosis in acute liver injury by TAA in rat,and the mechanisms in part were associated with FADD.

关 键 词：急性肝衰竭 硫代乙酰胺 银杏叶提取物 Fas相关死亡结构域蛋白 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]