Eupenicillium sp. E-UN41产活性物质的分离与结构分析(英文)  

Isolation and Structure Elucidation of an Active Metabolite from Eupenicillium sp.E-UN41

作　　者：杨煌建[1] 张祝兰[1] 任林英[1] 唐文力[1] 郑卫[1] 

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福州350007

出　　处：《天然产物研究与开发》2012年第9期1172-1175,共4页Natural Product Research and Development

基　　金：supported by the funds of national major programs on new drug creation during the 11th Five-Year Plan Period(2010ZX09401-403);Science and Technology Planning Project of Fujian Province(2009R10003-3)

摘　　要：在筛选微生物来源代谢产物的过程中,分离得到一株正青霉Eupenicillium sp. E-UN41,发酵液经离子交换树脂和葡聚糖凝胶等方法分离得到化合物UN41A。通过核磁共振波谱和X-单晶衍射进行该化合物的分子构型和晶体结构分析,确定其为4-氨甲酰基-1-β-D-呋喃核糖-5-羟基咪唑,与咪唑立宾同质。晶体属斜方晶系,空间群P212121,晶胞参数a=7.4897(7),b=11.3801(15),c=14.0090(13),V=1194.0(2)3,Z=4,Dc=1.553g/m3,F(000)=592。晶体结构分析表明,分子中存在咪唑环共平面。Eupenicillium sp.E-UN41 was obtained in the course of screening for bioactive microbial products,the compound UN41A was isolated by column chromatography of ion exchange resin and Sephadex G10,and purified by crystallization.The configuration and stereochemistry structure of UN41A were determined by NMR spectra and single crystal X-ray diffraction.UN41A was proved to be 4-carbamoyl-1-B-D-ribofuranosyl imidazolium-5-olate,the same structure as the immunosuppressant,mizoribine.The crystal of UN41A belonged to the orthorhombic P212121 space group with a = 7.4897（7）,b = 11.3801（15）,c = 14.0090（13）,V = 1194.0（2）3,Z=4,Dc=1.553g/m3,F（000） =592.The crystal of UN41A revealed that there was a imidazole ring plane.

关 键 词：正青霉 分离纯化 晶体结构 咪唑立宾 

分 类 号：Q939.97[生物学—微生物学] R979.5[医药卫生—药品]