杜仲绿原酸对高脂小鼠血液流变学作用研究  

Effect of Chlorogenic Acid Extracted from Eucommia ulmoides Oliv. on Hemorheological Changes of Mice Induced by High Fat Diet

作　　者：王建辉[1,2] 刘永乐[1] 李赤翎[1] 俞健[1] 王发祥[1] 李向红[1] 李艳 

机构地区：[1]长沙理工大学食品与生物工程系,长沙410114 [2]长沙高新开发区大自然生物科技有限公司,长沙410205

出　　处：《天然产物研究与开发》2012年第9期1186-1190,1200,共6页Natural Product Research and Development

基　　金：第39批留学回国人员科研启动基金项目;湖南省自然科学基金项目(11JJ6025);湖南省科技厅科技计划项目(2010NK3004);湖南省教育厅科学研究项目(10C0391)

摘　　要：旨以研究杜仲绿原酸对高脂高胆固醇诱导的高血脂模型小鼠血液流变学的影响,以昆明小鼠为实验动物,随机分成5组:阴性对照组,模型对照组和低剂量(25 mg/kg BW)、中剂量(50 mg/kg BW)、高剂量(100 mg/kg BW)杜仲绿原酸组,每组10只。后4组饲以高脂饲粮,同时小鼠灌胃杜仲绿原酸4周,实验结束,分别测定各组小鼠血液流变学参数、血清和肝脏的抗氧化酶活性和脂质过氧化产物MDA含量及其总抗氧化能力和羟自由基清除率。高脂血症小鼠的全血粘度、血浆粘度、红细胞压积、血沉、纤维蛋白原、红细胞刚性指数和聚集指数显著降低(P<0.05),红细胞变形指数显著提高(P<0.05),小鼠血清和肝脏SOD、GSH-Px水平、总抗氧化能力和羟自由基清除能力均显著升高(P<0.05),MDA水平显著降低(P<0.05)。在高脂膳食条件下,杜仲绿原酸能有效提高血液的抗氧化防御功能(包括抗氧化力、抗氧化酶活性)、改变血液流变学参数等,降低血液粘度、红细胞刚性和聚集,增强变形能力,使细胞膜的流动性增高,其中以中剂量效果相对较好。To investigate the effect of chlorogenic acid（CGA） extracted from Eucommia ulmoides Oliv.on blood hemorheological changes in hyperlipemia mice induced by high fat diet,fifty Kunming mice were randomly divided into 5 groups：negative control group,model control group,low dose（25 mg/kg BW）,medium dose（50 mg/kg BW）,and high dose（100 mg/kg BW） groups of CGA.The latter 4 groups were fed with high fat feed.CGA was given by gavage for 4 weeks.At the end of experiment,blood viscosity,plasma viscosity,hematocrit,erythrocyte indices of deformability,rigidity and aggregation,erythrocyte sedimentation rate and fibrinogen were assayed respectively.The antioxidant enzyme activities,MDA content,total antioxidant capabilities and hydroxyl radical scavenging rates in serum and liver were also measured.The blood viscosity,plasma viscosity,fibrinogen,erythrocyte sedimentation rate,erythrocyte indices of aggregation and rigidity were significantly reduced（P0.05）,erythrocyte index of deformability was significantly increased（P0.05）.The serum and liver SOD,GSH-Px level,total antioxidant capability and hydroxyl radical scavenging capacity were significantly increased（P0.05）,MDA production was significantly reduced（P0.05）.CGA extracted from E.ulmoides can effectively regulate the blood rheological parameters,and improve blood antioxidant capabilities and antioxidant enzyme activities.Through reduction in blood viscosity and erythrocyte of rigidity and aggregation,and enhancement in erythrocyte of deformability,CGA can improve the fluidity of cell membrane.Among them,the group at the dose of 50 mg/kg bw showed a relatively good effect.

关 键 词：杜仲 绿原酸 血液流变学变化 抗氧化 高血脂模型 

分 类 号：R258[医药卫生—中医内科学]