短毛切梢小蠹微卫星富集文库的构建与分析  

Construction and Analysis of Enriched Microsatellite Library of Tomicus brevipilosus

作　　者：曹荟[1] 朱家颖[1] 杨璞[2] 赵宁[1] 杨斌[1] 

机构地区：[1]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]中国林业科学研究院资源昆虫研究所,云南昆明650224

出　　处：《西南林业大学学报（自然科学）》2012年第5期62-65,共4页Journal of Southwest Forestry University：Natural Sciences

基　　金：西南林业大学科学研究基金面上项目(111128)资助;林业公益性行业科研专项(201004067)资助

摘　　要：采用生物素-磁珠富集法,构建短毛切梢小蠹微卫星富集文库。在该文库中随机挑选100个克隆作测序分析,结果表明:具微卫星位点的克隆占91%;具有10次以上碱基重复次数的微卫星序列占36%,其中最高碱基重复次数为63次;在这些微卫星序列中,完美型占47.3%,非完美型占24.2%,混合型占16.5%。表明获得的微卫星文库是高质量的文库,完美型的比例高于不完美型和混合型,与真核生物微卫星序列特征相符。The enriched microsatellite library of Tomicus brevipilosus （Eggers） was constructed using biotin-magnetic bead enrichment method. One hundred clones randomly chosen from the library were sequenced. The results showed that 91% of microsateUite loci were obtained. Among which the microsatellite sequences with more than 10 times of alkaline base repetitions accounted for 36%, and the highest alkaline base repetition was 63 times. From these microsatellites, 47.3% （about 43 repeat motifs） were perfect, 24.2% （about 22 repeat motifs） were imperfect, and 16.5% （about 15 repeat motifs） were compound. The results indicated that the enriched microsatellite library was of high-quality, in which the proportion of perfect microsatellites types was higher than that of the imperfect and compound types, which was in accordance with the feature of microsatellite sequences of other eukaryote.

关 键 词：短毛切梢小蠹 微卫星 文库构建 序列分析 

分 类 号：S718.7[农业科学—林学]