干扰素α缓释微球的制备及体外释放研究  

The Preparation and Research of Vitro-release of Interferon-loaded Microspheres

作　　者：孟乐乐[1] 洪晓芸[1] 张奇昕[1] 吴造展[1] 张丽君[1] 廖美玲[1] 张向荣 袁伟恩[1] 

机构地区：[1]上海交通大学药学院,上海200240 [2]深圳赛保尔生物药业有限公司,广东深圳518129

出　　处：《现代生物医学进展》2012年第27期5201-5203,5254,共4页Progress in Modern Biomedicine

基　　金：国家重大专项-"重大新药创制"科技重大专项-创新药物研究开发技术平台(2009ZX09310-007);国家自然科学基金(81173001);上海科委纳米专项(No11nm0503300和No.1052nm03900)

摘　　要：目的:开发一种有效地长效缓释干扰素α微球制剂。方法:利用S/O/W乳剂-挥发法制备了包裹干扰素α多糖颗粒的PLAG微球,对其外观形态进行了考察,并用ELISA方法考察了微球体外释放效果。结果:制备的干扰素α微球圆整光滑,粒径均匀;经24天体外释放,累计释放率达到80%以上。结论:通过包封包裹干扰素α的多糖颗粒进PLGA微球,有效地保护了干扰素α在微球中的活性,实现了长效缓释,是一种可行的缓释方案。Objective： To develop an effective sustained-release preparation of interferon a. Methods： Interferon a （IFN a）-loaded dextran nanoparticles were prepared by the method of freezing-induced phrase separation, then the IFNa-loaded dextran nanoparticles were microencapsulated in PLGA microspheres by the method of solid-in-oil-in-water （S/O/W） emulsion-evaporation technique. The microsphere samples were analyzed by using SEM. In vitro release profiles of the samples were detected by Elisa Kit. Results： The IFN-loaded microspheres had spherical shape and smooth surface. They possessed a normal size distribution. The accumulated release in vitro was over 80% after 24 days. Conclusion： The preparation of PLGA microspheres of IFN a through the encapsulation of IFN a-loaded Dextran nanoparticles protected the bioactivity of the IFN a in the microspheres effectively and got an ideal sustained release profile. This method is an effective technique for IFN a sustained release.

关 键 词：干扰素Α 长效缓释 体外释放 

分 类 号：R944.2[医药卫生—药剂学]