辛伐他汀对IFN-γ诱导的人外周血单个核细胞OX40L表达的影响  

The Effect of Simvastatin on Levels of OX40L in Human Peripheral Blood Mononuclear Cells(PBMCs)

作　　者：刘彬[1] 王凤军[1] 苏丹颖[1] 张惊宇[1] 陈修芬[1] 于美婷[1] 初婷婷[1] 刘欧[1] 侯丹慧[1] 张双彦[1] 

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2012年第27期5230-5233,共4页Progress in Modern Biomedicine

基　　金：黑龙江省科技攻关项目(2009-2012);合同编号:GC10C305-2

摘　　要：目的:探讨经IFN-γ刺激后,人外周血单个核细胞OX40L表达的变化,以及辛伐他汀对单个核细胞OX40L表达的影响。方法:将实验标本随机分为2组,分别干扰素-γ(IFN-γ)刺激组、辛伐他汀干预组。应用RT-PCR及Western blotting技术,观察IFN-γ诱导的人外周血单个核细胞OX40L表达情况及辛伐他汀对人单个核细胞OX40L表达的影响。结果:1.1000 U/ml IFN-与人单个核细胞共同培养24h后,OX40L mRNA和蛋白水平的表达明显增加。2.预先给予10 mol/L的辛伐他汀干预1h可以明显降低IFN-诱导的OX40L表达。结论:IFN-可诱导人单个核细胞OX40L表达。辛伐他汀可以抑制IFN-诱导人单个核细胞OX40L表达的增强,从而可能抑制了OX40L信号通路介导的与炎症有关的血管损伤,延缓动脉粥样硬化的进程。Objective： To observe the effect of simvastatin on serum levels of soluble OX40L （sOX40L） in PBMCs. Methods： Human peripheral blood mononuclear cells （PBMCs） were isolated, and the effects of simvastatin on OX40L expression were evaluated by RT-PCR and western blot. Results： OX40L mRNA and protein levels increased after stimulation with 1000 U/ml IFN-where simvastatin could antagonize this increase at a concentration of 10 mol/L. Conclusions： Simvastatin treatment induced down-regulation of OX40L mRNA and protein expression in a concentration-dependent manner in human peripheral blood mononuclear cells （PBMCs）. IFN-up-regulates the expression of the mRNA and protein of OX40L and this up-regulation could be reversed in present of simvastatin.

关 键 词：辛伐他汀 OX40配体 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]