大肠杆菌变种的两种分子生物学检测方法分析  

Analysis of Escherichia Coli Variants of the Molecular Biology Detection Methods

作　　者：曾婷[1] 马丽[1] 谢逸欣[1] 郝丽[1] 金星[1] 

机构地区：[1]广东省广州军区疾病预防控制中心,广东广州510507

出　　处：《现代生物医学进展》2012年第27期5238-5240,5400,共4页Progress in Modern Biomedicine

摘　　要：目的:对大肠杆菌的一种重要的变种--肠出血性大肠杆菌O157-H7的几种检测方法进行比较研究。方法:以自动免疫磁珠收集系统(AIMS)、自动酶标免疫测试系统(VIDAS)与传统常规的分离方法进行对比分析。结果:运用自动免疫磁珠收集系统(AIMS)方法对80份可能含有肠出血性大肠杆菌O157-H7的实样进行检测,检出份数为6份,检出率为7.5%,而且在一周之内可以全部对上述检出实样进行鉴定。AIMS法能够检出浓度在10cfu/mL模拟实样之中的肠出血性大肠杆菌O157-H7,然后将此法与CHROMagar 0157琼脂板相结合,其效果则更为明显。而自动酶标免疫测试系统(VIDAS)与传统与的分离方法则检测的效果不佳,检出率为0。自动免疫磁珠收集系统(AIMS)检测方法与自动酶标免疫测试系统(VIDAS)、传统与的分离方法在检出率方面存在显著的统计学差异,P<0.01。结论:运用自动免疫磁珠收集系统(AIMS)结合CHROMagar 0157琼脂板对出血性大肠杆菌O157-H7进行检测,检出率较高、灵敏度较高且快速便捷,可以用于O157-H7外环境检测与食品污染源的实际调查之中,应该对其加以广泛地推广并应用。Objective： Escherichia coli is a kind of important variants -- enterohemorrhagic Escherichia coli O157-H7 several detection methods are comparative study. Methods： The automated immunomagnetic beads collection system （ AIMS ）, automatic enzyme immunoassay test system （ VIDAS ） and traditional and separation method for comparative analysis. Results： The use of automated immunomagnetic beads collection system （ AIMS ） on80may contain enterohemorrhagic Escherichia coli O157-H7samples for detection, detection of copy number is 6, the detection rate was 7.5%, and within a week to all of the above can be detected samples for identification. AIMS method can detect concentration at 10cfu / mL simulation samples of enterohemorrhagic Escherichia coli O 157-H7, then this method and CHROMagar 0157agar plate combination, the effect is more obvious. Automatic enzyme immunoassay test system （ VIDAS ） and traditional and separation method detection effect is poor, the detection rate is 0. Conclusion： The use of automated immunomagnetic beads collection system （ AIMS ） combined with CHROMagar0157agar plates for hemorrhagic Escherichia coli O 157-H7were detected, the detection rate is higher, higher sensitivity and fast and convenient, can be used in O 157-H7environment detection and food contamination sources of practical investigation, should be widely promoted and applied.

关 键 词：肠出血性大肠杆菌O157-H7 自动免疫磁珠收集系统 自动酶标免疫测试系统 

分 类 号：R378[医药卫生—病原生物学] R446.1[医药卫生—基础医学]