用凝胶电泳发现肝素与染料之间的相互作用  

Using gel Electrophoresis Found the Interaction between Heparin and Dyes

作　　者：杨文杰[1] 韩丽红[1] 高丽君[2] 王步云[1] 秦文斌[2] 

机构地区：[1]包头医学院生物化学教研室,内蒙古包头014040 [2]包头医学院血红蛋白研究室,内蒙古包头014040

出　　处：《现代生物医学进展》2012年第27期5261-5264,共4页Progress in Modern Biomedicine

摘　　要：目的:观察肝素与23种染料之间的相互作用。方法:利用淀粉琼脂糖凝胶电泳及聚丙烯酰胺凝胶电泳,观察染料加肝素后电泳行为的变化。结果:淀粉琼脂糖凝胶电泳中染料加肝素后多数结果是泳速变慢,有一些留在原点,也有前移、后退者。二氯荧光素、荧光红、荧光素、甲酚红、苯胺蓝、溴酚蓝、茜素红S、胭脂红、亮绿、氨基黑10B、丽春红G、曙红、苯胺黑、氯酚红加肝素后泳速变慢。亚甲蓝、灿烂甲酚蓝、甲基紫加肝素后电泳留在原点。刚果红、洋红加肝素后电泳变化不明显。四氯荧光素、氯化硝基四氮唑蓝加肝素后电泳荧光看不清。聚丙烯酰胺凝胶电泳中溴酚蓝加入肝素后泳速明显变慢,随着肝素量增加溴酚兰的泳速加快。与淀粉琼脂糖凝胶电泳结果一致,但它的电泳结果更明显。结论:本次所研究的23种染料,几乎都能与肝素发生相互作用,给它们的进一步深入研究打下基础。Objective： To investigate the relationship between heparin and 23 kinds of dyes. Methods： Starch agarose gel electrophoresis and polyacrylamide gel electrophoresis were used to detect the changes of electrophoresis behavior after dye plus heparin. Results： Most electrophoresis results were slower than before after plus heparin, there were some stay in origin, also had moved forward, backward by starch agarose gel electrophoresis. The electrophoresis speed of dichlorofluorescein, fluorescent red, fluorescein, cresol red, aniline blue, bromophenol blue, the Sin purple S, carmine, light green, amido black 10B, Ponceau G, eosin, aniline black, chlorophenol red after plus heparin became slower. Methylene blue, brilliant cresyl blue, methyl violet after plus heparin electrophoresis stay at the origin. The electrophoresis of congo red, magenta after plus heparin did not change significantly. There were no electrophoresis fluorescence for tetrachloro-fluorescein, nitro tetrazolium blue after plus heparin. In polyacrylamide gel electrophoresis of bromophenol blue after adding heparin electrophoresis speed slowed down than before obviously, with the amount of heparin increased bromophenol blue electrophoresis speed up. Results of bromophenol blue were consistent with the starch agarose gel electrophoresis,but its electrophoresis results are more obvious. Conclusion： The study of 23 kinds of dyes, almost can interact with heparin.

关 键 词：电泳 肝素 染料 相互作用 

分 类 号：R96[医药卫生—药理学]