LH750血液分析仪白细胞分类结果异常的分析  

Analysis on abnormality of white blood cell differential count detected by LH750 hematological analyzer

作　　者：张丽霞 陆琳 刘健 周芸 高丽 潘世扬 

机构地区：[1]南京医科大学第一附属医院检验学部,南京210029

出　　处：《检验医学与临床》2012年第19期2401-2402,共2页Laboratory Medicine and Clinic

基　　金：江苏省实验诊断学重点实验室开放课题(XK201114);江苏高校优势学科建设工程资助项目

摘　　要：目的解决LH-750血液分析仪白细胞分类中单核细胞和嗜碱性粒细胞假性增高的干扰。方法对32例存在白细胞分类散点图异常以及血涂片手工分类证实存在单核细胞、嗜碱性粒细胞假性增高的EDTA-K2抗凝全血标本,在不同的放置时间用LH-750血液分析仪分别检测。结果适当延长标本放置时间,单核细胞和嗜碱性粒细胞百分比显著下降,接近手工分类值。结论合理的EDTA抗凝剂浓度以及适当的标本放置时间可以减少LH-750血液分析仪白细胞分类错误。Objective To resolve the interference of false increase of monocyte and basophilic granulocyte in white blood cell（WBC） differential count detected by the LH750 hematological analyzer.Methods 32 whole blood specimens anticoagulated by EDTA-K2 with abnormal WBC differential scatter diagram and false increase of monocyte and basophilic granulocyte confirmed by blood smear manual classification were detected after different storage time by the LH750 hematological analyzer.Results After properly prolonging the storage time of specimens,the percentage of monocyte and basophile granulocyte were significantly decreased and closed to the manual differential count values.Conclusion The rational EDTA anticoagulant concentration and suitable specimen storage time can reduce the error of WBC differential count detected by the LH750 hematological analyzer.

关 键 词：样本放置时间 抗凝剂浓度 单核细胞 嗜碱性粒细胞 

分 类 号：R446.111[医药卫生—诊断学]