胶束毛细管电泳在线推扫法测定中成药中的士的宁  

Determination of strychnine in Chinese patent medicine by on-line sweeping proconcentration method in capillary electrophoresis

作　　者：王竞[1] 吴敏[1] 孙成均[2] 徐小平[1] 李章万[1] 钱广生[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学华西公共卫生学院,四川成都610041

出　　处：《华西药学杂志》2012年第5期560-562,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家"十一五"科技支撑计划课题资助(课题号:2006BAD27B02)

摘　　要：目的采用胶束毛细管电泳在线推扫法测定中成药中的微量士的宁。方法运行缓冲液为50 mmol.L-1NaH2PO4-90 mmol.L-1SDS-乙腈(83∶83∶34),pH2;0.5 psi压力进样,进样时间3.7 min;分离电压25 kV;分离温度25℃;检测波长205 nm。结果士的宁0.5～8.0μg.mL-1峰面积线性关系良好(r=0.9998),检测限达0.020μg.mL-1;方法回收率>97.6%;日内、日间RSD均小于1.8%。结论所用方法快速、准确,有效提高了毛细管电泳法的灵敏度。OBJECTIVE To establish an on-line sweeping proconcentration method in capillary electrophoresis for determination of the trace strychnine in Chinese patent medicine.METHODS On-line sweeping proconcentration method in capillary electrophoresis was used with running buffer of 50 mmol·L-1 NaH2PO4-90 mmol·L-1 SDS-acetonitrile（83：83：34,pH2,injection time of 3.7 min at 0.5 psi and an applied voltage of 25 kV at 25 ℃.The detection wavelength was 205 nm.RESULTS The linearity of strychnine was obtained over the range of 0.5-8.0 μg·mL-1（r=0.9998） and the detection limit was 0.020 μg·mL-1.The mean recovery of this method ranged form 97.6% to 103.4%.The intra and inter day precision of strychnine was no more than 1.8%.CONCLUSION This method is rapid,accurate and effectively improved the sensitivity of capillary electrophoresis.

关 键 词：胶束毛细管电泳在线推扫 中成药 士的宁 

分 类 号：R917[医药卫生—药物分析学]