HPLC测定蜀五加和糙叶藤五加中的金丝桃苷  

Determination of hyperoside in Acanthopanax setchuenensis and Acanthopanax leucorrhizus var.fulvescens by HPLC

作　　者：李莹[1,2,3] 左旭[1] 李艳丹[1] 刘圆[1] 夏清[2] 

机构地区：[1]西南民族大学民族医药研究院,四川成都610041 [2]四川中医药高等专科学校,四川绵阳621000 [3]成都中医药大学药学院,四川成都611137

出　　处：《华西药学杂志》2012年第5期576-578,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家科技支撑计划项目(2012BAI27B07);四川省科技支撑计划(2011SZ0233);四川省杰出青年学术技术带头人后续计划(2011JQ0051);四川省中医药管理局科技专项(2008-32)

摘　　要：目的采用LC-MS定性鉴别蜀五加和糙叶藤五加中的金丝桃苷;采用HPLC测定蜀五加和糙叶藤五加中金丝桃苷的含量,并比较两种五加不同药用部位的金丝桃苷含量。方法采用Kromasil C18柱(150 mm×4.6 mm,5μm),柱温30℃,流动相以甲醇(A)和0.5%磷酸水溶液(B),梯度洗脱,检测波长358 nm,流速1.0 mL.min-1。结果金丝桃苷0.0250～1.25μg与峰面积具有良好的线性关系,回归方程为:Y=4×106X+1.327×104(r=0.9999),平均回收率为100.25%,RSD=0.96%(n=9)。结论所用方法简便、准确、重复性好,可用于蜀五加和糙叶藤五加药材质量控制方法的研究。OBJECTIVE To determine hyperoside of Acanthopanax setchuenensis Harms ex Diels and A.leucorrhigus var.fulvescens Harms Rehd by LC-MS.And to establish a RP-HPLC method to determine the hyperoside content from different medical parts of those two plants.METHODS The analysis was carried out at 30 ℃ on a Kromasil C18 column eluted with a mobile phase consisted of methanol（A）-0.5% phosphoric acid（B）.The detective wavelength was at 358 nm with the flow rate of 1.0 mL·min-1.RESULTS The calibration curve was linear within the concentration range of 0.025-1.25 μg with r of 0.9999.The average recovery was 100.25% with RSD of 0.96%（n=9）.CONCLUSION This method was simple,accurate and replicable.It was useful for the study of the quality control of A.setchuenensis Harms ex Diels and A.leucorrhigus var.fulvescens Harms Rehd.

关 键 词：蜀五加 糙叶藤五加 金丝桃苷 LC-MS 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]