乙酰半胱氨酸对^(238)Pu α粒子致肺癌模型活性氧和表型影响  

Effect of acetyl cysteine on reactive oxygen species level and phenotype of malignant BERP35T-1 cells exposed to ^(238)Pu α-particles

作　　者：佟鹏[1] 王春燕[1] 魏志权[1] 齐雪松[1] 郝述霞[1] 苟巧[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所毒理学研究室,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《中国职业医学》2012年第5期369-372,379,共5页China Occupational Medicine

基　　金：国家自然科学基金资助项目(81000862)

摘　　要：目的研究N-乙酰半胱氨酸(NAC)对钚-238(238Pu)α粒子辐射致肺癌细胞模型BERP35T-1细胞内活性氧自由基(ROS)水平以及细胞增殖和迁移的影响。方法用不同浓度NAC(0～10 mmol/L)作用BERP35T-1细胞72 h后,以及BERP35T-1细胞经1 mmol/L NAC作用不同时间(0～72 h)后,二苯基溴化四氮唑蓝(MTT)法检测细胞增殖情况;DCFH-DA荧光探针标记结合荧光显微镜观察实验和细胞划痕愈合实验分别检测人支气管上皮细胞BEP2D、BERP35T-1细胞以及1 mmol/L NAC作用BERP35T-1 48 h后细胞内ROS水平和细胞的迁移能力。结果 1～10 mmol/LNAC作用BERP35T-1 72 h后,以及1 mmol/L NAC作用BERP35T-1 24～72 h后,细胞增殖受到显著抑制(P<0.01)。与BEP2D细胞比较,BERP35T-1细胞内ROS水平明显升高(P<0.01),细胞的迁移能力明显增强(P<0.01);1 mmol/LNAC作用BERP35T-1 48 h后,细胞内ROS水平显著降低(P<0.01),细胞的迁移能力显著减弱(P<0.01)。结论抗氧化剂NAC可显著抑制人支气管上皮细胞α粒子辐射致癌模型BERP35T-1的恶性增殖和迁移,作用机制可能与其降低细胞内基础ROS水平有关。Objective To study the effect of acetyl cysteine （NAC） on the intracellular reactive oxygen species （ROS） level,proliferation and migration of malignant BERP35T-1 cells exposed to 23SPu α-particles. Methods BERP35T-1 cells were treated with 0 - 10 mmol/L NAC for 72 hours or 1 mmol/L NAC for 0 - 72 hours, then the proliferation of BERP35T-1 cells were examined with MTF assay. With DCHF-DA, the generation of ROS in human bronchial epithelial cell line BEP2D and BERP35T-1 treated with and without 1 mmol/L NAC for 48 hours were monitored by the fluorescence microscope. The migration abilities of BEP2D and BERP35T-I treated with and without 1mmol/L NAC for 48 hours were determined by scratch healing as- say. Results After treated with 1 - 10 mmol/L NAC for 72 hours or 1 mmol/L NAC for 24 -72 hours, the proliferation of BERP35T-1 cells were significantly inhibited （P 〈 0. 01 ）. Compared with those of BEP2D cells, greatly increased intracellular ROS levels and migration ability of BERP35T-1 cells were detected （ P 〈 0.01 ）. After treated with 1 mmol/L NAC for 48 hours, intraeellular ROS levels and migration ability of BERP35T-1 cells decreased significantly （ P 〈 0. 01 ）. Conclusion NAC, as an antioxidant, may inhibit the malignant proliferation and metastasis of malignant BERP35T-1 cells exposed to or-particles through reducing intracellular ROS levels.

关 键 词：N-乙酰半胱氨酸 Α粒子 肺癌 增殖 迁移 

分 类 号：X591[环境科学与工程—环境工程]