硫酸铍致小鼠肝线粒体功能障碍研究  

Study on beryllium sulfate on mice liver mitochondrial dysfunction

作　　者：张朝晖[1] 王淑娟[1] 骆金俊[1] 黄炼[1] 李程[1] 廖永红[1] 

机构地区：[1]南华大学公共卫生学院环境医学与放射卫生研究所,湖南衡阳421001

出　　处：《中国职业医学》2012年第5期376-379,共4页China Occupational Medicine

基　　金：湖南省科技厅资助项目(2011TT2013);湖南省研究生科研创新项目(CX2011B382);衡阳市科技局资助课题(2011ks11)

摘　　要：目的探讨硫酸铍(BeSO4.4H2O)对小鼠肝线粒体功能的影响。方法将36只6周龄昆明(KM)小鼠随机分为对照组、低剂量组及高剂量组3组,每组12只,雌雄各半。对照组采用灭菌生理氯化钠溶液按0.01 ml/g(体质量)隔天腹腔注射,低、高剂量组分别以1、2 mg/kg BeSO4.4H2O生理氯化钠溶液隔天腹腔注射染毒,连续染毒3周。取肝脏采用差速离心法制备线粒体,采用荧光分光光度法测定其活性氧(ROS)水平及线粒体膜电位(Δψm),采用分光光度法测定细胞色素C(Cytc)水平及线粒体肿胀程度。结果与对照组比较,低、高剂量组肝脏系数增高,肝脏线粒体ROS及Cytc水平随染毒剂量的增加而升高,差异均有统计学意义(P<0.05);随染毒剂量的增加Δψm下降、线粒体肿胀明显。结论 BeSO4.4H2O可致小鼠肝线粒体功能障碍。Objective To explore the effect of beryllium sulfate （BeSO4· 4H2O） on mice liver mitochondrial tunctinn. Methods 36 KM mice were randomly divided into 3 groups and administered with saline solution of beryllium sulfate （ l and 2 mg/kg） by intraperitoneal injection for 3 weeks. Liver mitochondria were isolated by differential centlifugation, then the level of mitoehondrial reactive oxygen species （ROS） and mitochondrial cytochrome c （Cytc） , mitoehondrial swelling and the mitochon- drial membrane potential （AtOm） were detected. Results The relative organ weight of liver in exposure groups were significantly higher than those of the controls （P 〈 0. 05 ）. Compared with those nf the control group, ROS and Cyte in all exposed groups were significantly higher （ P 〈 0. 05 ）. A dramatic mitochondrial swelling and a decrease of △ψm in the dose groups were found. Conclusion Beryllium sulfate can induce mitochondrial dysfunction on mice liver mitochondrial.

关 键 词：硫酸铍 线粒体功能障碍 活性氧 细胞色素C 跨膜电位 

分 类 号：R114[医药卫生—卫生毒理学]