哈蟆油非水溶性成分及其酶解物的抗氧化功效研究  

Anti-oxidation of oviductus ranae water-insoluble part and hydrolysate

作　　者：瞿新明[1] 李芸彤[1] 张惠[1] 张鑫[1] 王思明[1] 赵雨[1] 

机构地区：[1]长春中医药大学中医药与生物工程研发中心,长春130117

出　　处：《食品科技》2012年第10期211-213,共3页Food Science and Technology

基　　金：国家"十一五"科技支撑项目(2007BAI38B06)

摘　　要：研究哈蟆油非水溶性成分及其酶解物的抗氧化作用。采用D-半乳糖法建立小鼠过氧化损伤模型。设立哈蟆油非水溶性成分及其酶解物高、中、低(0.30、0.15、0.075g/kg)3个剂量组和模型对照组、空白对照组,灌胃给药30d。观察过氧化损伤模型小鼠肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力的变化。哈蟆油非水溶性成分可以显著降低肝组织中MDA含量,但对SOD、GSH-Px活力的升高无影响;哈蟆油非水溶性成分酶解物可显著降低丙二醛(MDA)含量,显著增加肝组织GSH-Px、SOD的活力。哈蟆油非水溶成分酶解物具有明显的抗氧化作用。Studied on anti-oxidation of oviductus ranae water-insoluble part and it＇s hydrolysate. Established D-half of lactose mice＇s injury models. Set high, medium and low three doses （0.075, 0.15, 0.30 g/kg） of oviductus ranae water-insoluble part and it＇s hydrolysate and a blank dose had been applied to mice who were treated for 30 days continuously. Observed liver malonaldehyde （MDA）＇s content, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activity of changes. Experimental results show that oviductus ranae water-insoluble part can obviously reduce the MDA, but for SOD, GSH-Px had not significantly increased. Water-insoluble part＇s hydrolysate can significantly reduce content of MDA, significantly increase activity of GSH-Px and SOD. Oviductus ranae water-insoluble part hydrolysates have obvious antioxidant effects.

关 键 词：哈蟆油非水溶性成分 哈蟆油非水溶性成分酶解物 抗氧化 

分 类 号：R285.5[医药卫生—中药学]