红树植物老鼠簕抗肝损伤有效部位化学成分分离及含量测定  

Chemical Constituents Isolation and Content Determination of Effective Parts of Anti-liver Injury of Mangrove Acanthus Ilicifoliusl

作　　者：陈朝霞[1] 刘军[1] 陈胡兰[1] 张梅[1] 

机构地区：[1]成都中医药大学药学院 中药材标准化教育部重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《成都中医药大学学报》2012年第3期42-44,52,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家海洋局海洋生物活性物质与现代分析技术重点实验室开放基金资助项目(编号:MBSMAT-2009-04)

摘　　要：目的:对老鼠簕抗肝损伤有效部位—正丁醇提取物化学成分进行分离,并测定不同产地老鼠簕中2-苯并噁唑啉酮的含量。方法:采用常压反复硅胶柱层析方法,结合反相硅胶、Sephadex LH-20等分离材料进行分离纯化;采用反相高效液相色谱法(RP-HPLC)测定2-苯并噁唑啉酮含量。结果:从老鼠簕正丁醇提取物中分离鉴定了6个单体成分,7个不同产地的老鼠簕中2-苯并噁唑啉酮的平均含量为0.4249 mg/g。结论:研究结果为阐明老鼠簕抗肝损伤药效物质基础,完善其质量控制方法提供了一定的实验依据。Objective： To isolate the chemical constituents of effective parts of anti-liver injury-butanol extract, and to deter- mine the content of 2-benzoxazolinone of Acanthus llicifoliusl in different areas. Methods： The compounds were isolated and purified by atnmspherie pressure silica gel eulumn chromatography and some separation materials （ reverse phase silica gel, Sephedex LH-20） ; And the RP-HPLC was used to determine the content of 2-benzoxazolinone. Results： Six compounds were isolated and identified from n-butyl alcohol extract of Acanthus Ilicifoliusland, and we got the result that the average content of 2-benzoxazolinone from 7 different districts was 0. 425mg/g. Conclusion： The research provided a scientific base for clarifying the material basis of anti-liver injury and the quality control method of Acanthus ilicifolius.

关 键 词：老鼠簕 化学成分 含量测定 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]