芩术四物汤中黄芩苷和芍药苷的含量测定  

Determination of Baicalin and Paeoniflorin in Qin-zhu-si-wu Decoction

作　　者：黄道秋[1] 贺钢民[2] 李柏群[1] 彭腾[2] 邓赟[2] 邱建平[2] 李鸿翔[2] 

机构地区：[1]重庆三峡中心医院药剂科,重庆万州404000 [2]成都中医药大学中药材标准化教育部重点实验室,四川成都611137

出　　处：《成都中医药大学学报》2012年第3期45-47,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：重庆市科委科技攻关资助项目(编号:CSTC;2011AC5141)

摘　　要：目的:建立芩术四物汤中黄芩苷、芍药苷的含量测定方法,控制其质量。方法:采用Dia-monsil C18(250 mm×4.6 mm,5 m)色谱柱;流动相分别为甲醇-水-磷酸(47∶53∶0.2),乙腈-水-磷酸(14∶86∶0.1);检测波长为280 nm及230 nm;流速:1.0 mL/min;柱温:25℃。结果:复方中黄芩苷在0.03147～1.007 mg/mL范围内线性关系良好,回归方程为Y=3E-08X-0.0037(r=0.9999),平均回收率为98.17%,RSD%=1.14%。芍药苷在0.03116～0.997 mg/mL范围内线性关系良好,回归方程为Y=8E-0.8X+0.0011(r=0.9999),平均回收率为97.83%,RSD%=1.24%。结论:该方法简便、快速、准确、重复性好,为芩术四物汤的质量控制提供了理论依据。Objective： To establish the assay methods for paeoniflorin and baicalin acid in Qin-Zhu-si-wu Decoction, control- ling its quality. Methods： The contents of baicalin and paeoniflorin were analyzed by HPLC. Chromatographic conditions included： Diamonsil Cls （250 mm x4.6 nun, 5 μm） column and the mobile phase consisted of a mixture of methanol-water-phosphoric acid （47： 53： 0. 2）, aeetonitrile-water-phosphoric acid （ 14： 86： 0. 1 ）. Baicalin and paeoniflorin acid were detected at 280 nm and 230 nm respectively, and the flow rate was 1.0 ml/min. Column temperature is 25℃. Results： Tile linear range was from 0. 03147 to 1. 007 mg/ml for Baicalin. Regression equation ： Y = 3E-08X-0. 0037 （ r = 0. 9999） ; The average recovery was 98. 17 %, RSD = 1.14 % The linear range was from 0. 03116 to 0. 997mg/ml for Paeoniflorin. Regression equation ： Y = 8E - 0. 8X ＋ 0. 0011 （ r = 1 ） ; The average recovery was 97. 83%, RSD = 1.24%. Conclusion： The method wag simple, rapid, precise and repeatable. This method Can be used for controlling the quality of Qinzhusiwu Decoctio.

关 键 词：芩术四物汤 黄芩苷 芍药苷 高效液相 

分 类 号：R289.3[医药卫生—方剂学] R284.1[医药卫生—中药学]