DDRT-PCR分析铁高效和铁低效小麦品种基因表达的差异  

Difference of Gene Expression in Wheat Roots under Iron-Sufficient and Iron-Deficient Conditions by DDRT-PCR

作　　者：印莉萍[1] 刘维仲[1] 刘祥林[1] 柴小清[1] 邱泽生 张福锁[2] 

机构地区：[1]首都师范大学生物系 [2]中国农业大学

出　　处：《首都师范大学学报（自然科学版）》2000年第2期58-63,共6页Journal of Capital Normal University：Natural Science Edition

基　　金：北京市自然科学基金资助!(5 942 0 0 4和 5 982 0 0 8)

摘　　要：利用mRNA差异显示 (DifferentialDisplayReverseTranscriptPCR ,简称DDRT PCR)技术比较了正常供和缺铁胁迫下铁高效型小麦 (京— 4 11)的铁低效型小麦 (三属麦— 3)的基因表达差异模式 .此差异模式暗示出两个品种表型差异的遗传背景 .利用挑选出的差异显示cDNA片段为探针进行Northern杂交 ,证明一些差异cDNA的表达受缺铁胁迫的抑制 .测序和序列分析结果表明 ,受抑制的cDNA片段分别与Cytc基因、ATP binding transporters基因、和dDTP 4 ,6 葡萄糖脱水酶的基因有一定同源性 .Expression difference of gene under iron\|deficiency and iron\|efficiency were compared between iron\|efficient wheat cultivar Jing—411 and iron\|inefficient wheat cultivar Sanshumai—3 by differential display Reverse Transcript PCR (DDRT\|PCR) method. Northern blotting were carried out with root tips of wheat cultival Jing—411 which treated by iron\|deficiency stress and iron\|efficiency, and the probes come from the special cDNA fragment selected from DDRT\|PCR products of the same cultival. The results demonstrated some cDNAs expression were suppressed in iron\|deficiency condition. Sequened analysis showed that cDNA fragment were homogenous to Cytochrome C gene\,ATP\|binding transporters (ABC transporters) gene and dTDP\|D\|glucose 4,6\|dehydratase.

关 键 词：铁高效小麦 DDRT-PCR 铁低效小麦 品种 基因表达 

分 类 号：Q753[生物学—分子生物学] S512.101[农业科学—作物学]