牡丹‘乌龙捧盛’鳞芽组织培养技术研究  

Study on Tissue Culture of Peony Sprout

作　　者：刘磊[1] 黄镶[1] 仲照东[2] 卢莉 

机构地区：[1]信阳农业高等专科学校园艺系,河南信阳464000 [2]信阳农业高等专科学校规划设计系,河南信阳464000 [3]信阳市第六高级中学,河南信阳464100

出　　处：《北方园艺》2012年第20期115-116,共2页Northern Horticulture

摘　　要：以牡丹鳞芽为试材,分别研究了不同暗处理时间和植物生长调节剂组合对鳞芽褐化与生根的影响。结果表明:在鳞芽初代培养过程中,先暗培养7d,而后转入正常光照培养,可明显减轻鳞芽褐化;诱导根原基形成的最适培养基为:1/2MS+NAA 2.0mg/L+IAA 2.0mg/L+6-BA 0.1mg/L或1/2MS+NAA 1.0mg/L+IBA 1.0mg/L+AC 0.2g/L。The effects of different dark treatments and plant growth regulators combination on browning and rooting were studied respectively.The results showed that during the course of initial culture,sprout was cultured under dark for 7 d,and then sprout was cultured under normal light condition,and browning was relieved obviously.The most optimum medium for root primordium was 1/2MS＋NAA 2.0 mg/L＋IAA 2.0 mg/L＋6-BA 0.1 mg/L or 1/2MS＋NAA 1.0 mg/L＋IBA 1.0 mg/L＋AC 0.2 g/L.

关 键 词：牡丹 鳞芽 组织培养 

分 类 号：S685.110.36[农业科学—观赏园艺]