“童子一号”草莓玻璃化法超低温保存技术研究  

Study on Cryo-preservation Technology with Vitrification of 'Tongzi No.1' Strawberry

作　　者：徐启红[1] 任平国[1] 

机构地区：[1]漯河职业技术学院,河南漯河462000

出　　处：《北方园艺》2012年第20期117-119,共3页Northern Horticulture

基　　金：河南省高等学校青年骨干教师计划资助项目(2011GGJS-278)

摘　　要：以玻璃化法进行"童子一号"草莓离体茎尖的超低温保存研究,探讨了低温锻炼时间、预培养时间、预处理时间、PVS2处理时间等对存活率的影响。结果表明:将4℃条件下低温锻炼30d的健壮草莓苗茎尖,接种在固体预培养培养基中预培养4d,预处理30min后,吸出预处理液,加入PVS2,0℃处理80min后,更换新鲜的PVS2,并迅速投入液氮60min后,置于40℃快速化冻1min;室温洗涤3次并取出茎尖,接种到再生培养基恢复培养;成活率最高可达43.00%,且再生植株分化正常。Researching on the ＇Tongzi No.1＇strawberry meristem-tip cryo-preservation with vitrification,the effect of various factors of vitrification,such as,low temperature acclimatization time,pre-culture time,pretreatment time,PVS2 treatment time,were studied on survival rate of strawberry meristem-tip after cryo-preservation.The results showed that after 30 d and acclimatizing strawberry meristem-tips at 4℃,they were cultured in solid preculture medium after 4 d;pretreat for 30 min,suck up the pre-culture medium,and then put them into PVS2 at 0℃ for 80 min;then put them in the fresh PVS2,pre-serve in LN for 1 h;and then throw them rapidly into water at 40℃ for 1 min;wash the shoot tips 3 times at room temperature and then plant the tips to regeneration medium.The survival rate could reach the highest about 43% and rege-nerative plants become divided regularly.

关 键 词：草莓 超低温保存 玻璃化 

分 类 号：S668.409.3[农业科学—果树学]